8CR3
Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-24 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.68880 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.542, 89.875, 41.340 |
| Unit cell angles | 90.00, 113.96, 90.00 |
Refinement procedure
| Resolution | 28.590 - 1.120 |
| R-factor | 0.1622 |
| Rwork | 0.162 |
| R-free | 0.17010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.938 | 1.136 |
| High resolution limit [Å] | 1.116 | 1.116 |
| Rmerge | 0.071 | 0.565 |
| Rmeas | 0.086 | 0.683 |
| Rpim | 0.048 | 0.380 |
| Number of reflections | 190922 | 5145 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.994 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M magnesium formate, 20% (w/v) PEG 3350 |
