8CMV

Engineered PETase enzyme from LCC - C09 mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leaf-branch compost cutinase	polymer	301	32759.0	1	UniProt (G9BY57) Pfam (PF12695)	unidentified prokaryotic organism	LC-cutinase,LCC,PET-digesting enzyme,Poly(ethylene terephthalate) hydrolase,PET hydrolase,PETase
2	B (A)	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
3	C, D, E, F (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	G (A)	water	water		18.0	248	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: G9BY57)

PDB	External Database	Details
Asp 36	Ser 36	conflict
Arg 40	Gln 40	conflict
Asp 48	Ser 48	conflict
Lys 57	Ser 57	conflict
Gly 127	Tyr 127	conflict
Arg 145	Ser 145	conflict
Arg 209	Ala 209	conflict
Cys 238	Asp 238	conflict
Ile 243	Phe 243	conflict
Asp 276	Asn 276	conflict
Lys 278	Asn 278	conflict
Cys 283	Ser 283	conflict
Lys 293	Gln 293	engineered mutation
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32759.0
Non-Polymers*	Number of molecules	5
	Total formula weight	428.5
All*	Total formula weight	33187.5

*Water molecules are not included.