8CMV

Engineered PETase enzyme from LCC - C09 mutant

Summary for 8CMV

• Entry DOI: 10.2210/pdb8cmv/pdb
• Descriptor: Leaf-branch compost cutinase, ISOPROPYL ALCOHOL, GLYCEROL, ... (4 entities in total)
• Functional Keywords: plastic degradation, thermal stablization, mhet, bhet, tpa, hydrolase
• Biological source: unidentified prokaryotic organism
• Total number of polymer chains: 1
• Total formula weight: 33187.52

Authors

Bhattacharya, S.,Estiri, H.,Castagna, R.,Parisini, E. (deposition date: 2023-02-21, release date: 2024-03-06, Last modification date: 2026-02-18)

Primary citation

Bhattacharya, S.,Castagna, R.,Estiri, H.,Upmanis, T.,Ricci, A.,Gautieri, A.,Parisini, E.
Development of a highly active engineered PETase enzyme for polyester degradation.
Febs J., 293:443-455, 2026

PubMed Abstract: Polyethylene terephthalate (PET) accounts for ≈6% of global plastic production, contributing considerably to the global solid-waste stream and environmental plastic pollution. Since the discovery of PET-depolymerizing enzymes, enzymatic PET recycling has been regarded as a promising method for plastic disposal, particularly in the context of a circular economy strategy. However, because the PET-degrading enzymes developed so far suffer from relatively limited thermostability and low catalytic efficiency, as well as degradation product inhibition, their large-scale industrial applications are still largely hampered. To overcome these limitations, we engineered the current PET-hydrolyzing enzyme gold standard [the ICCG variant of leaf-branch compost cutinase (LCC-ICCG)] using in silico protein design methods to develop a PET-hydrolyzing enzyme that features enhanced thermal stability and PET depolymerization activity. Our mutant, LCC-ICCG-C09, features a 3.5 °C increase in melting temperature relative to the LCC-ICCG enzyme. Under optimal reaction conditions (68 °C), the engineered enzyme hydrolyzes amorphous PET material into terephthalic acid (TPA) with a two-fold higher efficiency compared to LCC-ICCG. Owing to its enhanced properties, LCC-ICCG-C09 may be a promising candidate for future applications in industrial PET recycling processes.

PubMed: 40847613
DOI: 10.1111/febs.70228

Experimental method

X-RAY DIFFRACTION (1.28 Å)