8CI2

human alpha7 nicotinic receptor in complex with the C4 nanobody under sub-saturating conditions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Neuronal acetylcholine receptor subunit alpha-7	polymer	388	44050.9	5	UniProt (P36544) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)
2	F, G, H (F, G, I)	Nanobody C4	polymer	147	15826.4	3		Vicugna pacos
3	I, J, K, L, M (P, Q, R, S, T)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
4	N, O, P, Q, R... (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)

Sequence modifications

A, B, C, D, E: 1 - 324 (UniProt: P36544)
A, B, C, D, E: 328 - 375 (UniProt: P36544)

PDB	External Database	Details
Asp 325	-	linker
Gly 326	-	linker
Val 327	-	linker
Ser 376	-	expression tag
Ala 377	-	expression tag
Gly 378	-	expression tag
Leu 379	-	expression tag
Thr 380	-	expression tag
Glu 381	-	expression tag
Thr 382	-	expression tag
Ser 383	-	expression tag
Gln 384	-	expression tag
Val 385	-	expression tag
Ala 386	-	expression tag
Pro 387	-	expression tag
Ala 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	267733.9
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	10
	Total formula weight	2212.1
All*	Total formula weight	272068.0

*Water molecules are not included.