8C8J
Long Interspersed Nuclear Element 1 (LINE-1) reverse transcriptase ternary complex with hybrid duplex and dTTP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | RNA-directed DNA polymerase | polymer | 832 | 98020.7 | 1 | UniProt (G8I2S9) Pfam (PF00078) UniProt (by SIFTS) (O00370) In PDB | Homo sapiens (human) | |
2 | B | DNA (5'-D(P*GP*CP*GP*CP*TP*TP*TP*CP*C)-3') | polymer | 9 | 2682.8 | 1 | synthetic construct | ||
3 | C | RNA (5'-R(P*UP*UP*AP*GP*GP*AP*AP*AP*GP*CP*GP*C)-3') | polymer | 12 | 3875.4 | 1 | synthetic construct | ||
4 | A | CITRIC ACID | non-polymer | 192.1 | 2 | Chemie (CIT) | |||
5 | A | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
6 | A, C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 14 | Chemie (EDO) | |||
7 | A | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 2 | Chemie (DMS) | |||
8 | A | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 1 | Chemie (DIO) | |||
9 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
10 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
11 | water | water | 18.0 | 219 | Chemie (HOH) |
Sequence modifications
A: 238 - 1061 (UniProt: G8I2S9)
PDB | External Database | Details |
---|---|---|
Asp 434 | Asn 434 | conflict |
His 910 | Leu 910 | conflict |
His 1062 | - | expression tag |
His 1063 | - | expression tag |
His 1064 | - | expression tag |
His 1065 | - | expression tag |
His 1066 | - | expression tag |
His 1067 | - | expression tag |
His 1068 | - | expression tag |
His 1069 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 104578.8 | |
Non-Polymers* | Number of molecules | 23 |
Total formula weight | 2063.8 | |
All* | Total formula weight | 106642.6 |