8C8J
Long Interspersed Nuclear Element 1 (LINE-1) reverse transcriptase ternary complex with hybrid duplex and dTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-05 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.349, 84.528, 107.938 |
Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
Resolution | 59.660 - 2.100 |
R-factor | 0.2093 |
Rwork | 0.208 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.890 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.8 (8-JUN-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.660 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.018 | 0.427 |
Number of reflections | 62715 | 4676 |
<I/σ(I)> | 18.7 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 41.2 | 42.1 |
CC(1/2) | 1.000 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |
2 | VAPOR DIFFUSION | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |
3 | VAPOR DIFFUSION | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |
4 | VAPOR DIFFUSION | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |
5 | VAPOR DIFFUSION | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |
6 | VAPOR DIFFUSION | 5.6 | 293 | PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide |