8BW9
Cryo-EM structure of the RAF activating complex KSR-MEK-CNK-HYP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein aveugle | polymer | 110 | 13143.9 | 1 | UniProt (Q8ML92) Pfam (PF07647) | Drosophila melanogaster (fruit fly) | |
| 2 | B (B) | Connector enhancer of KSR protein CNK | polymer | 335 | 38534.2 | 1 | UniProt (Q7KNQ9) Pfam (PF00536) Pfam (PF10534) Pfam (PF00595) | Drosophila melanogaster (fruit fly) | Connector enhancer of ksr,isoform A,isoform B,SD09985p |
| 3 | C (C) | Dual specificity mitogen-activated protein kinase kinase dSOR1 | polymer | 396 | 43926.5 | 1 | UniProt (Q24324) Pfam (PF00069) | Drosophila melanogaster (fruit fly) | Downstream of RAF,MAPKK |
| 4 | D (D) | KSR | polymer | 316 | 36474.9 | 1 | UniProt (Q24170) Pfam (PF07714) UniProt (by SIFTS) (Q24171) | Drosophila melanogaster (fruit fly) | |
| 5 | E (C) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 6 | F (C) | Trametinib | non-polymer | 615.4 | 1 | Chemie (QOM) | |||
| 7 | G (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 2 - 106 (UniProt: Q8ML92)
B: 1 - 330 (UniProt: Q7KNQ9)
D: 654 - 966 (UniProt: Q24170)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Pro 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Met -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 651 | - | expression tag |
| Gly 652 | - | expression tag |
| Arg 653 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 132079.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1145.9 | |
| All* | Total formula weight | 133225.4 |
