8BW9

Cryo-EM structure of the RAF activating complex KSR-MEK-CNK-HYP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein aveugle	polymer	110	13143.9	1	UniProt (Q8ML92) Pfam (PF07647) In PDB	Drosophila melanogaster (fruit fly)
2	B	Connector enhancer of KSR protein CNK	polymer	335	38534.2	1	UniProt (Q7KNQ9) Pfam (PF00536) Pfam (PF10534) Pfam (PF00595) In PDB	Drosophila melanogaster (fruit fly)	Connector enhancer of ksr,isoform A,isoform B,SD09985p
3	C	Dual specificity mitogen-activated protein kinase kinase dSOR1	polymer	396	43926.5	1	UniProt (Q24324) Pfam (PF00069) In PDB	Drosophila melanogaster (fruit fly)	Downstream of RAF,MAPKK
4	D	KSR	polymer	316	36474.9	1	UniProt (Q24170) Pfam (PF07714) UniProt (by SIFTS) (Q24171) In PDB	Drosophila melanogaster (fruit fly)
5	C	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
6	C	Trametinib	non-polymer		615.4	1	Chemie (QOM)
7	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 2 - 106 (UniProt: Q8ML92)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Asp 0	-	expression tag
Pro 1	-	expression tag

B: 1 - 330 (UniProt: Q7KNQ9)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

D: 654 - 966 (UniProt: Q24170)

PDB	External Database	Details
Gly 651	-	expression tag
Gly 652	-	expression tag
Arg 653	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132079.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1145.9
All*	Total formula weight	133225.4

*Water molecules are not included.