8BSU
Crystal structure of the kainate receptor GluK3-H523A ligand binding domain in complex with kainate and the positive allosteric modulator BPAM344 at 2.9A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Glutamate receptor ionotropic, kainate 3 | polymer | 258 | 29025.4 | 8 | UniProt (P42264) Pfam (PF10613) Pfam (PF00060) | Rattus norvegicus (Norway rat) | GluK3,Glutamate receptor 7,GluR-7,GluR7 |
| 2 | EA, I, NB, OA, T... (C, A, F, D, B...) | 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide | non-polymer | 242.3 | 8 | Chemie (2J9) PubChem (67531324) | |||
| 3 | AB, BB, CB, DB, EC... (E, H, C, A, F...) | ZINC ION | non-polymer | 65.4 | 20 | Chemie (ZN) | |||
| 4 | EB, FB, GB, GC, HA... (E, H, C, A, D...) | GLYCEROL | non-polymer | 92.1 | 13 | Chemie (GOL) PubChem (753) | |||
| 5 | AA, AC, BC, HC, IC... (B, G, H, C, E...) | CHLORIDE ION | non-polymer | 35.5 | 22 | Chemie (CL) PubChem (312) | |||
| 6 | BA, CC, KC, LB, P... (B, G, H, E, A...) | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 7 | CA, DC, LC, MB, NA... (B, G, H, E, C...) | 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE | non-polymer | 213.2 | 8 | Chemie (KAI) PubChem (23616889) PubChem (45039619) PubChem (101994997) PubChem (135037246) PubChem (6603893) PubChem (3816) PubChem (10255) PubChem (73345) PubChem (3083828) PubChem (5317452) PubChem (5318769) PubChem (6453379) PubChem (6604705) PubChem (10036135) PubChem (11095940) | |||
| 8 | DA, S, VB (B, A, F) | ACETATE ION | non-polymer | 59.0 | 3 | Chemie (ACT) PubChem (175) | |||
| 9 | MC, NC, OC, PC, QC... (A, B, C, D, E...) | water | water | 18.0 | 115 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 4 - 118 (UniProt: P42264)
A, B, C, D, E, F, G, H: 121 - 258 (UniProt: P42264)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Ala 95 | His 523 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 119 | - | linker |
| Thr 120 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 232203.1 | |
| Non-Polymers* | Number of molecules | 82 |
| Total formula weight | 7875.0 | |
| All* | Total formula weight | 240078.1 |
