8BSU

Crystal structure of the kainate receptor GluK3-H523A ligand binding domain in complex with kainate and the positive allosteric modulator BPAM344 at 2.9A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Glutamate receptor ionotropic, kainate 3	polymer	258	29025.4	8	UniProt (P42264) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Norway rat)	GluK3,Glutamate receptor 7,GluR-7,GluR7
2	EA, I, NB, OA, T... (C, A, F, D, B...)	4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		242.3	8	Chemie (2J9)
3	AB, BB, CB, DB, EC... (E, H, C, A, F...)	ZINC ION	non-polymer		65.4	20	Chemie (ZN)
4	EB, FB, GB, GC, HA... (E, H, C, A, D...)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL)
5	AA, AC, BC, HC, IC... (B, G, H, C, E...)	CHLORIDE ION	non-polymer		35.5	22	Chemie (CL)
6	BA, CC, KC, LB, P... (B, G, H, E, A...)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
7	CA, DC, LC, MB, NA... (B, G, H, E, C...)	3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE	non-polymer		213.2	8	Chemie (KAI)
8	DA, S, VB (B, A, F)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
9	MC, NC, OC, PC, QC... (A, B, C, D, E...)	water	water		18.0	115	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 4 - 118 (UniProt: P42264)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Gly 3	-	expression tag
Ala 95	His 523	engineered mutation

A, B, C, D, E, F, G, H: 121 - 258 (UniProt: P42264)

PDB	External Database	Details
Gly 119	-	linker
Thr 120	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	232203.1
Non-Polymers*	Number of molecules	82
	Total formula weight	7875.0
All*	Total formula weight	240078.1

*Water molecules are not included.