8BJI

chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Actin, alpha skeletal muscle, intermediate form	polymer	375	41862.6	1	UniProt (P68135) Pfam (PF00022) In PDB	Oryctolagus cuniculus (rabbit)
2	B	Putative Adenylate cyclase,Profilin-1	polymer	591	65232.9	1	UniProt (A0A6N3LUE9) UniProt (P07737) Pfam (PF03497) Pfam (PF00235) UniProt (by SIFTS) (A0A9P1NJI6) In PDB	Vibrio nigripulchritudo	MARTX toxin,Epididymis tissue protein Li 184a,Profilin I
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A, B	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
6	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
8	B, A	HYDROGEN PEROXIDE	non-polymer		34.0	14	Chemie (PEO)
9	B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
10		water	water		18.0	944	Chemie (HOH)

Sequence modifications

B: 455 - 895 (UniProt: A0A6N3LUE9)

PDB	External Database	Details
Gly 451	-	expression tag
Pro 452	-	expression tag
Gly 453	-	expression tag
Ser 454	-	expression tag
Pro 896	-	linker
Pro 897	-	linker
Pro 898	-	linker
Pro 899	-	linker
Pro 900	-	linker
Gly 901	-	linker
Gly 902	-	linker

B: 903 - 1041 (UniProt: P07737)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	107095.5
Non-Polymers*	Number of molecules	27
	Total formula weight	2040.8
All*	Total formula weight	109136.3

*Water molecules are not included.