8BJI
chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.974, 62.854, 93.588 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 25.060 - 1.750 |
| R-factor | 0.1772 |
| Rwork | 0.175 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pav |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS (Version Jan 31, 2020 BUILT=20200131) |
| Data scaling software | Aimless (Version 0.7.4) |
| Phasing software | PHASER (Version 2.8.3) |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.810 | 1.820 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.123 | 1.094 |
| Number of reflections | 83837 | 4192 |
| <I/σ(I)> | 8.5 | 1.6 |
| Completeness [%] | 94.6 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 30% peg 4000, 0.2 M Lithium Sulfate (LiSO4), 0.1 M TrisHCl pH8.5 |
