8BJI
chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.974, 62.854, 93.588 |
Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
Resolution | 25.060 - 1.750 |
R-factor | 0.1772 |
Rwork | 0.175 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pav |
RMSD bond length | 0.008 |
RMSD bond angle | 0.920 |
Data reduction software | XDS (Version Jan 31, 2020 BUILT=20200131) |
Data scaling software | Aimless (Version 0.7.4) |
Phasing software | PHASER (Version 2.8.3) |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.810 | 1.820 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.123 | 1.094 |
Number of reflections | 83837 | 4192 |
<I/σ(I)> | 8.5 | 1.6 |
Completeness [%] | 94.6 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293 | 30% peg 4000, 0.2 M Lithium Sulfate (LiSO4), 0.1 M TrisHCl pH8.5 |