8B41

Structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Volume-regulated anion channel subunit LRRC8A	polymer	817	95056.3	4	UniProt (Q80WG5) Pfam (PF12534) Pfam (PF13855)	Mus musculus (house mouse)	Leucine-rich repeat-containing protein 8A,Protein ebouriffe,ebo
2	E, F (E, F)	Volume-regulated anion channel subunit LRRC8C	polymer	811	93472.6	2	UniProt (Q8R502) Pfam (PF12534) Pfam (PF13855)	Mus musculus (house mouse)	Factor for adipocyte differentiation 158,Leucine-rich repeat-containing protein 8C
3	G, H, I, J (G, H, I, J)	Synthetic nanobody Sb1	polymer	118	13115.5	4		Synthetic construct

Sequence modifications

A, B, C, D: 2 - 810 (UniProt: Q80WG5)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Leu 811	-	expression tag
Glu 812	-	expression tag
Val 813	-	expression tag
Leu 814	-	expression tag
Phe 815	-	expression tag
Gln 816	-	expression tag

E, F: 2 - 803 (UniProt: Q8R502)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Arg 781	Gly 781	conflict
Ala 804	-	expression tag
Leu 805	-	expression tag
Glu 806	-	expression tag
Val 807	-	expression tag
Leu 808	-	expression tag
Phe 809	-	expression tag
Gln 810	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	619632.6
All*	Total formula weight	619632.6

*Water molecules are not included.