8AP1

Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with two GTP molecules poised for de novo initiation (IC2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Mitochondrial transcription factor 1	polymer	354	41151.2	1	UniProt (P14908) Pfam (PF00398) In PDB	Saccharomyces cerevisiae S288C	Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023
2	A	DNA-directed RNA polymerase, mitochondrial	polymer	1262	143282.7	1	UniProt (P13433) Pfam (PF14700) Pfam (PF00940) In PDB	Saccharomyces cerevisiae S288C
3	N	Non-template DNA (33-MER)	polymer	33	10248.7	1		Saccharomyces cerevisiae S288C
4	T	Template DNA (33-MER)	polymer	33	10047.5	1		Saccharomyces cerevisiae S288C
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	A, T	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

B: 2 - 341 (UniProt: P14908)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

A: 100 - 1351 (UniProt: P13433)

PDB	External Database	Details
Gly 90	-	expression tag
Ala 91	-	expression tag
Met 92	-	expression tag
Gly 93	-	expression tag
Ser 94	-	expression tag
Gly 95	-	expression tag
Ile 96	-	expression tag
Gln 97	-	expression tag
Arg 98	-	expression tag
Pro 99	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	204730.0
Non-Polymers*	Number of molecules	3
	Total formula weight	1070.7
All*	Total formula weight	205800.7

*Water molecules are not included.