8AP1

Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with two GTP molecules poised for de novo initiation (IC2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000002	biological_process	mitochondrial genome maintenance
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0001018	molecular_function	mitochondrial promoter sequence-specific DNA binding
A	0003677	molecular_function	DNA binding
A	0003896	molecular_function	DNA primase activity
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006269	biological_process	DNA replication, synthesis of primer
A	0006351	biological_process	DNA-templated transcription
A	0006390	biological_process	mitochondrial transcription
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0034245	cellular_component	mitochondrial DNA-directed RNA polymerase complex
A	0042645	cellular_component	mitochondrial nucleoid
A	0043231	cellular_component	intracellular membrane-bounded organelle
B	0003677	molecular_function	DNA binding
B	0003723	molecular_function	RNA binding
B	0005739	cellular_component	mitochondrion
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005759	cellular_component	mitochondrial matrix
B	0006390	biological_process	mitochondrial transcription
B	0006391	biological_process	transcription initiation at mitochondrial promoter
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation
B	0032786	biological_process	positive regulation of DNA-templated transcription, elongation
B	0034245	cellular_component	mitochondrial DNA-directed RNA polymerase complex
B	0034246	molecular_function	mitochondrial transcription factor activity

Functional Information from PROSITE/UniProt

site_id	PS00489
Number of Residues	15
Details	RNA_POL_PHAGE_2 Bacteriophage-type RNA polymerase family active site signature 2. ItRkvvKqtVMTnvY

Chain	Residue	Details
A	ILE1008-TYR1022

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site_id	PS00900
Number of Residues	12
Details	RNA_POL_PHAGE_1 Bacteriophage-type RNA polymerase family active site signature 1. PVhqDGTCNGLQ

Chain	Residue	Details
A	PRO941-GLN952

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	ASP945
A	LYS1014
A	ASP1189
B	ASN137

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