7ZTA

Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	16S1	16S ribosomal RNA	polymer	1534	497405.0	1	GenBank (1108560344)	Escherichia coli K-12
2	S021	30S ribosomal protein S2	polymer	224	24971.8	1	UniProt (P0A7V0) Pfam (PF00318) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS2
3	S031	30S ribosomal protein S3	polymer	206	23078.8	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS3
4	S041	30S ribosomal protein S4	polymer	205	23383.0	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS4
5	S051	30S ribosomal protein S5	polymer	155	16361.9	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS5
6	S061	30S ribosomal protein S6, fully modified isoform	polymer	106	12326.3	1	UniProt (P02358) Pfam (PF01250) In PDB	Escherichia coli K-12
7	S071	30S ribosomal protein S7	polymer	151	16861.5	1	UniProt (P02359) Pfam (PF00177) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS7
8	S081	30S ribosomal protein S8	polymer	129	14015.4	1	UniProt (P0A7W7) Pfam (PF00410) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS8
9	S091	30S ribosomal protein S9	polymer	127	14554.9	1	UniProt (P0A7X3) Pfam (PF00380) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS9
10	S101	30S ribosomal protein S10	polymer	99	11325.1	1	UniProt (P0A7R5) Pfam (PF00338) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS10,Transcription termination/antitermination protein NusE
11	S111	30S ribosomal protein S11	polymer	117	12487.2	1	UniProt (P0A7R9) Pfam (PF00411) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS11
12	S121	30S ribosomal protein S12	polymer	123	13683.1	1	UniProt (P0A7S3) Pfam (PF00164) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS12
13	S131	30S ribosomal protein S13	polymer	114	12625.8	1	UniProt (P0A7S9) Pfam (PF00416) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS13
14	S141	30S ribosomal protein S14	polymer	101	11546.4	1	UniProt (P0AG59) Pfam (PF00253) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS14
15	S151	30S ribosomal protein S15	polymer	88	10159.6	1	UniProt (P0ADZ4) Pfam (PF00312) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS15
16	S161	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886) In PDB	Escherichia coli K-12	Small ribosomal subunit protein bS16
17	S171	30S ribosomal protein S17	polymer	80	9263.9	1	UniProt (P0AG63) Pfam (PF00366) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS17
18	S181	30S ribosomal protein S18	polymer	65	7606.8	1	UniProt (P0A7T7) Pfam (PF01084) In PDB	Escherichia coli K-12	Small ribosomal subunit protein bS18
19	S191	30S ribosomal protein S19	polymer	83	9421.0	1	UniProt (P0A7U3) Pfam (PF00203) In PDB	Escherichia coli K-12	Small ribosomal subunit protein uS19
20	S201	30S ribosomal protein S20	polymer	86	9577.3	1	UniProt (P0A7U7) Pfam (PF01649) In PDB	Escherichia coli K-12	Small ribosomal subunit protein bS20
21	S211	30S ribosomal protein S21	polymer	56	6629.7	1	UniProt (P68679) Pfam (PF01165) In PDB	Escherichia coli K-12	Small ribosomal subunit protein bS21
22	23S1	23S ribosomal RNA	polymer	2903	941505.4	1		Escherichia coli K-12
23	05S1	5S ribosomal RNA	polymer	120	38790.1	1	GenBank (1845258627)	Escherichia coli K-12
24	L021	50S ribosomal protein L2	polymer	271	29663.2	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL2
25	L031	50S ribosomal protein L3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL3
26	L041	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL4
27	L051	50S ribosomal protein L5	polymer	177	20073.2	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL5
28	L061	50S ribosomal protein L6	polymer	176	18801.6	1	UniProt (P0AG55) Pfam (PF00347) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL6
29	L091	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF03948) Pfam (PF01281) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL9
30	L131	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL13
31	L141	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL14
32	L151	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL15
33	L161	50S ribosomal protein L16	polymer	136	15327.3	1	UniProt (P0ADY7) Pfam (PF00252) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL16
34	L171	50S ribosomal protein L17	polymer	118	13505.7	1	UniProt (P0AG44) Pfam (PF01196) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL17
35	L181	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL18
36	L191	50S ribosomal protein L19	polymer	114	13028.1	1	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL19
37	L201	50S ribosomal protein L20	polymer	117	13396.8	1	UniProt (P0A7L3) Pfam (PF00453) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL20
38	L211	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL21
39	L221	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL22
40	L231	50S ribosomal protein L23	polymer	93	10546.5	1	UniProt (P0ADZ0) Pfam (PF00276) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL23
41	L241	50S ribosomal protein L24	polymer	102	11078.9	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL24
42	L251	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL25
43	L271	50S ribosomal protein L27	polymer	84	9015.3	1	UniProt (P0A7L8) Pfam (PF01016) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL27
44	L281	50S ribosomal protein L28	polymer	77	8896.4	1	UniProt (P0A7M2) Pfam (PF00830) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL28
45	L291	50S ribosomal protein L29	polymer	62	7155.3	1	UniProt (P0A7M6) Pfam (PF00831) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL29
46	L301	50S ribosomal protein L30	polymer	58	6423.6	1	UniProt (P0AG51) Pfam (PF00327) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL30
47	L311	50S ribosomal protein L31	polymer	66	7516.7	1	UniProt (P0A7M9) Pfam (PF01197) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL31-A
48	L321	50S ribosomal protein L32	polymer	56	6332.2	1	UniProt (P0A7N4) Pfam (PF01783) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL32
49	L331	50S ribosomal protein L33	polymer	51	5928.0	1	UniProt (P0A7N9) Pfam (PF00471) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL33
50	L341	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL34
51	L351	50S ribosomal protein L35	polymer	64	7181.8	1	UniProt (P0A7Q1) Pfam (PF01632) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL35,Ribosomal protein A
52	L361	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL36-A,Ribosomal protein B
53	ATR1	tRNA-Cys	polymer	74	23923.4	1	GenBank (1845826323)	Escherichia coli K-12
54	PTR1	tRNA-Lys	polymer	76	24617.8	1		Escherichia coli K-12
55	PQK1	MAAAPQK nascent peptide	polymer	7	716.9	1		Escherichia coli K-12
56	ETR1	tRNA-Gln	polymer	75	24134.5	1	GenBank (1847035720)	Escherichia coli K-12
57	MRN1	mRNA	polymer	14	4525.8	1		Escherichia coli K-12
58	16S1, 23S1, 05S1, L021, L031...	MAGNESIUM ION	non-polymer		24.3	289	Chemie (MG)
59	16S1, 23S1, S131, 05S1, L021	POTASSIUM ION	non-polymer		39.1	115	Chemie (K)
60	S021, L311, L361	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
61	23S1	Tetracenomycin X	non-polymer		486.4	1	Chemie (OCW)
62	ATR1	CYSTEINE	non-polymer		121.2	1	Chemie (CYS)
63	ETR1	GLUTAMINE	non-polymer		146.1	1	Chemie (GLN)
64		water	water		18.0	775	Chemie (HOH)

Sequence modifications

S141: 1 - 101 (UniProt: P0AG59)

PDB	External Database	Details
Ala 35	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	57
	Total formula weight	2190525.4
Non-Polymers*	Number of molecules	410
	Total formula weight	12470.4
All*	Total formula weight	2202995.8

*Water molecules are not included.