7ZRZ

Structure of the human tRNA splicing endonuclease defines substrate recognition

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AP1)	tRNA-splicing endonuclease subunit Sen34	polymer	263	28805.8	1	UniProt (Q9BSV6) Pfam (PF26577) Pfam (PF01974)	Homo sapiens (human)	Leukocyte receptor cluster member 5,tRNA-intron endonuclease Sen34,HsSen34
2	B (BP4)	tRNA-splicing endonuclease subunit Sen2	polymer	260	30166.0	1	UniProt (Q8NCE0) Pfam (PF02778) Pfam (PF01974)	Homo sapiens (human)	tRNA-intron endonuclease Sen2,HsSen2
3	C (CP1)	tRNA-splicing endonuclease subunit Sen54	polymer	293	32551.7	1	UniProt (Q7Z6J9) Pfam (PF12928)	Homo sapiens (human)	SEN54 homolog,HsSEN54,tRNA-intron endonuclease Sen54
4	D (DP1)	tRNA-splicing endonuclease subunit Sen15	polymer	217	23860.9	1	UniProt (Q8WW01) Pfam (PF09631)	Homo sapiens (human)	SEN15 homolog,HsSEN15,tRNA-intron endonuclease Sen15
5	E (ZN1)	pre-tRNA Arg TCT 3-2	polymer	89	28747.0	1		Homo sapiens

Sequence modifications

AP1: 1 - 167 (UniProt: Q9BSV6)
AP1: 180 - 310 (UniProt: Q9BSV6)

PDB	External Database	Details
Gly 168	-	linker
Gly 169	-	linker
Ser 170	-	linker
Gly 171	-	linker
Gly 172	-	linker
Ser 173	-	linker
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Phe 255	His 255	conflict

BP4: 206 - 286 (UniProt: Q8NCE0)
BP4: 292 - 465 (UniProt: Q8NCE0)

PDB	External Database	Details
Gly 287	-	linker
Gly 288	-	linker
Ser 289	-	linker
Gly 290	-	linker
Gly 291	-	linker
Phe 377	His 377	conflict

CP1: 1 - 411 (UniProt: Q7Z6J9)
CP1: 425 - 526 (UniProt: Q7Z6J9)

PDB	External Database	Details
Gly 412	-	linker
Gly 413	-	linker
Ser 414	-	linker
Gly 415	-	linker
Gly 416	-	linker
Ser 417	-	linker
Gly 418	-	linker
Gly 419	-	linker
Ser 420	-	linker
Gly 421	-	linker
Gly 422	-	linker
Ser 423	-	linker
Gly 424	-	linker

DP1: 1 - 171 (UniProt: Q8WW01)

PDB	External Database	Details
Met -45	-	initiating methionine
Asp -44	-	expression tag
Glu -43	-	expression tag
Lys -42	-	expression tag
Thr -41	-	expression tag
Thr -40	-	expression tag
Gly -39	-	expression tag
Trp -38	-	expression tag
Arg -37	-	expression tag
Gly -36	-	expression tag
Gly -35	-	expression tag
His -34	-	expression tag
Val -33	-	expression tag
Val -32	-	expression tag
Glu -31	-	expression tag
Gly -30	-	expression tag
Leu -29	-	expression tag
Ala -28	-	expression tag
Gly -27	-	expression tag
Glu -26	-	expression tag
Leu -25	-	expression tag
Glu -24	-	expression tag
Gln -23	-	expression tag
Leu -22	-	expression tag
Arg -21	-	expression tag
Ala -20	-	expression tag
Arg -19	-	expression tag
Leu -18	-	expression tag
Glu -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Gly -12	-	expression tag
Gln -11	-	expression tag
Arg -10	-	expression tag
Glu -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	144131.4
All*	Total formula weight	144131.4

*Water molecules are not included.