7ZPJ

Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endoribonuclease Dicer	polymer	2004	226925.7	1	UniProt (Q8R418) Pfam (PF04851) Pfam (PF20930) Pfam (PF00271) Pfam (PF03368) Pfam (PF20931) Pfam (PF02170) Pfam (PF00636) Pfam (PF20932) In PDB	Mus musculus (house mouse)	Double-strand-specific ribonuclease mDCR-1
2	B	59-nt precursor of miR-15a	polymer	59	19017.3	1	GenBank (262205589)	Mus musculus
3	D	RISC-loading complex subunit TARBP2 isoform 1	polymer	373	39991.1	1	UniProt (P97473) Pfam (PF00035) In PDB	Mus musculus (house mouse)	Protamine-1 RNA-binding protein,PRM-1 RNA-binding protein,TAR RNA-binding protein 2

Sequence modifications

A: 1 - 1916 (UniProt: Q8R418)

PDB	External Database	Details
Met -52	-	initiating methionine
Val -51	-	expression tag
Trp -50	-	expression tag
Ser -49	-	expression tag
His -48	-	expression tag
Pro -47	-	expression tag
Gln -46	-	expression tag
Phe -45	-	expression tag
Glu -44	-	expression tag
Lys -43	-	expression tag
Gly -42	-	expression tag
Gly -41	-	expression tag
Gly -40	-	expression tag
Ser -39	-	expression tag
Gly -38	-	expression tag
Gly -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
Gly -34	-	expression tag
Gly -33	-	expression tag
Ser -32	-	expression tag
Ala -31	-	expression tag
Trp -30	-	expression tag
Ser -29	-	expression tag
His -28	-	expression tag
Pro -27	-	expression tag
Gln -26	-	expression tag
Phe -25	-	expression tag
Glu -24	-	expression tag
Lys -23	-	expression tag
Gly -22	-	expression tag
Asp -21	-	expression tag
Tyr -20	-	expression tag
Pro -19	-	expression tag
Tyr -18	-	expression tag
Asp -17	-	expression tag
Val -16	-	expression tag
Pro -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Ala -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag
Ser 1110	Thr 1110	conflict
Ser 1619	Ala 1619	conflict
Gly 1917	-	expression tag
Arg 1918	-	expression tag
Gly 1919	-	expression tag
Glu 1920	-	expression tag
Asn 1921	-	expression tag
Leu 1922	-	expression tag
Tyr 1923	-	expression tag
Phe 1924	-	expression tag
Gln 1925	-	expression tag
Gly 1926	-	expression tag
Ala 1927	-	expression tag
Ser 1928	-	expression tag
Asp 1929	-	expression tag
Tyr 1930	-	expression tag
Lys 1931	-	expression tag
Asp 1932	-	expression tag
His 1933	-	expression tag
Asp 1934	-	expression tag
Gly 1935	-	expression tag
Asp 1936	-	expression tag
Tyr 1937	-	expression tag
Lys 1938	-	expression tag
Asp 1939	-	expression tag
His 1940	-	expression tag
Asp 1941	-	expression tag
Gly 1942	-	expression tag
Ser 1943	-	expression tag
His 1944	-	expression tag
His 1945	-	expression tag
His 1946	-	expression tag
His 1947	-	expression tag
His 1948	-	expression tag
His 1949	-	expression tag
His 1950	-	expression tag
His 1951	-	expression tag

D: 1 - 365 (UniProt: P97473)

PDB	External Database	Details
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	285934.0
All*	Total formula weight	285934.0

*Water molecules are not included.