7ZG0

Murine IL-27 in complex with IL-27Ra and a non-competing Nb

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Interleukin-27 subunit alpha	polymer	246	27762.8	2	UniProt (Q8K3I6)	Mus musculus (house mouse)	IL-27 subunit alpha,IL-27-A,IL27-A,p28
2	C, D (C, D)	Interleukin-27 subunit beta	polymer	241	26476.4	2	UniProt (O35228) Pfam (PF00041)	Mus musculus (house mouse)	IL-27 subunit beta,IL-27B,Epstein-Barr virus-induced gene 3 protein homolog
3	E, F (E, F)	Interleukin-27 receptor subunit alpha	polymer	219	25217.4	2	UniProt (O70394)	Mus musculus (house mouse)	IL-27 receptor subunit alpha,IL-27R subunit alpha,IL-27R-alpha,IL-27RA,Type I T-cell cytokine receptor,TCCR,WSX-1
4	G, H (G, H)	Nanobody 5	polymer	166	17611.5	2		Lama glama
5	I, J (I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
6	K, L, M, N (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
7	O, P, Q, R (A, B, C, D)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, B: 28 - 234 (UniProt: Q8K3I6)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ile 0	-	expression tag
Leu 1	-	expression tag
Pro 2	-	expression tag
Ser 3	-	expression tag
Pro 4	-	expression tag
Gly 5	-	expression tag
Met 6	-	expression tag
Pro 7	-	expression tag
Ala 8	-	expression tag
Leu 9	-	expression tag
Leu 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Ser 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Ser 17	-	expression tag
Val 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Met 21	-	expression tag
Gly 22	-	expression tag
Cys 23	-	expression tag
Val 24	-	expression tag
Ala 25	-	expression tag
Glu 26	-	expression tag
Thr 27	-	expression tag
Gly 235	-	expression tag
Thr 236	-	expression tag
Lys 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag

C, D: 18 - 228 (UniProt: O35228)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ile -10	-	expression tag
Leu -9	-	expression tag
Pro -8	-	expression tag
Ser -7	-	expression tag
Pro -6	-	expression tag
Gly -5	-	expression tag
Met -4	-	expression tag
Pro -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ser 1	-	expression tag
Leu 2	-	expression tag
Val 3	-	expression tag
Ser 4	-	expression tag
Leu 5	-	expression tag
Leu 6	-	expression tag
Ser 7	-	expression tag
Val 8	-	expression tag
Leu 9	-	expression tag
Leu 10	-	expression tag
Met 11	-	expression tag
Gly 12	-	expression tag
Cys 13	-	expression tag
Val 14	-	expression tag
Ala 15	-	expression tag
Glu 16	-	expression tag
Thr 17	-	expression tag

E, F: 24 - 225 (UniProt: O70394)

PDB	External Database	Details
Glu 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Glu 16	-	expression tag
Asn 17	-	expression tag
Leu 18	-	expression tag
Tyr 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag
Thr 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	194136.2
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	195869.9

*Water molecules are not included.