7ZG0

Murine IL-27 in complex with IL-27Ra and a non-competing Nb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	167.242, 137.707, 111.479
Unit cell angles	90.00, 117.49, 90.00

Refinement procedure

Resolution	79.520 - 3.180
R-factor	0.2319
Rwork	0.229
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rosettafold
Data reduction software	XDS (20210323)
Data scaling software	XDS (20210323)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.898	100.898	3.370
High resolution limit [Å]	3.175	9.490	3.175
Rmerge	0.175
Rmeas	0.189	0.048	1.555
Number of reflections	37561	1450	6029
<I/σ(I)>	9.28	34.39	1.3
Completeness [%]	98.9	98	98.4
Redundancy	7.028	6.71	7.01
CC(1/2)	0.997	0.999	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	Wizard screen H12 derived. 1 M Potassium sodium tartrate, 0.1 M MES/ Sodium hydroxide pH 5.5