7Z4D
Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F (A, F) | sgRNA | polymer | 84 | 27242.3 | 2 | synthetic construct | ||
| 2 | B, E (B, E) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 2 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 3 | C, G (C, G) | Target strand of 10 nucleotide complementary DNA substrate | polymer | 30 | 9149.9 | 2 | synthetic construct | ||
| 4 | D, H (D, H) | Non-target strand of 10 nucleotide complementary DNA substrate | polymer | 30 | 9222.0 | 2 | synthetic construct | ||
| 5 | I, J, K, L, M... (A, B, C, E, F...) | POTASSIUM ION | non-polymer | 39.1 | 16 | Chemie (K) | |||
| 6 | AA, BA, CA, DA, EA... (C, D, E, F, G...) | water | water | 18.0 | 88 | Chemie (HOH) |
Sequence modifications
B, E: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 408405.9 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 625.6 | |
| All* | Total formula weight | 409031.4 |
