7Z4D
Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004520 | molecular_function | DNA endonuclease activity |
| B | 0004527 | molecular_function | exonuclease activity |
| B | 0008408 | molecular_function | 3'-5' exonuclease activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051607 | biological_process | defense response to virus |
| E | 0003677 | molecular_function | DNA binding |
| E | 0003723 | molecular_function | RNA binding |
| E | 0004518 | molecular_function | nuclease activity |
| E | 0004519 | molecular_function | endonuclease activity |
| E | 0004520 | molecular_function | DNA endonuclease activity |
| E | 0004527 | molecular_function | exonuclease activity |
| E | 0008408 | molecular_function | 3'-5' exonuclease activity |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 34 |
| Details | Region: {"description":"ARM","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 177 |
| Details | Region: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 538 |
| Details | Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Motif: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"25079318","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 10 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
