7Z4D
Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.636, 95.509, 169.797 |
| Unit cell angles | 80.83, 78.04, 62.34 |
Refinement procedure
| Resolution | 66.490 - 3.100 |
| R-factor | 0.2479 |
| Rwork | 0.246 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fq5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.547 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.490 | 3.211 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.340 | 2.176 |
| Number of reflections | 85696 | 4963 |
| <I/σ(I)> | 7.62 | 1.31 |
| Completeness [%] | 89.2 | 56.74 |
| Redundancy | 10.2 | 10.1 |
| CC(1/2) | 0.997 | 0.446 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M sodium cacodylate pH 6.5, 0.8-1.2M ammonium formate, 12-14% PEG4000 |
