7Z3P

Crystal structure of the mouse leptin:LepR-CRH2 encounter complex to 1.95 A resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leptin	polymer	159	17340.7	1	UniProt (P41160) Pfam (PF02024)	Mus musculus (house mouse)	Obesity factor
2	B (B)	Leptin receptor	polymer	232	26038.5	1	UniProt (P48356) Pfam (PF18589)	Mus musculus (house mouse)	LEP-R,B219,OB receptor,OB-R
3	C (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	D (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	E, F (A, B)	water	water		18.0	154	Chemie (HOH)

Sequence modifications

A: 22 - 167 (UniProt: P41160)

PDB	External Database	Details
Ala 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Pro 16	-	expression tag
Gly 17	-	expression tag
Gly 18	-	expression tag
Pro 19	-	expression tag
Gly 20	-	expression tag
Gly 21	-	expression tag

B: 426 - 633 (UniProt: P48356)

PDB	External Database	Details
Ala 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
Pro 409	-	expression tag
Gly 410	-	expression tag
Gly 411	-	expression tag
Pro 412	-	expression tag
Gly 413	-	expression tag
Ser 414	-	expression tag
Glu 415	-	expression tag
Asn 416	-	expression tag
Leu 417	-	expression tag
Tyr 418	-	expression tag
Phe 419	-	expression tag
Gln 420	-	expression tag
Gly 421	-	expression tag
Gly 422	-	expression tag
Ser 423	-	expression tag
Ser 424	-	expression tag
Gly 425	-	expression tag
Gln 514	Asn 514	conflict
Ser 602	Cys 602	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43379.2
Non-Polymers*	Number of molecules	2
	Total formula weight	146.2
All*	Total formula weight	43525.4

*Water molecules are not included.