7Z3P

Crystal structure of the mouse leptin:LepR-CRH2 encounter complex to 1.95 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980110
Spacegroup name	P 21 21 2
Unit cell lengths	96.727, 52.811, 89.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.350 - 1.943
R-factor	0.1937
Rwork	0.191
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ax8 3v6o
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS (version March 15, 2019)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.7.0)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.350	1.990
High resolution limit [Å]	1.940	1.940
Rmeas	0.127	3.271
Number of reflections	34115	2017
<I/σ(I)>	16.08	0.8
Completeness [%]	99.2	88.9
Redundancy	13.2
CC(1/2)	0.999	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	BCS screen, condition H1 0.04 M Calcium chloride dihydrate 0.04 M sodium formate 0.1 M Tris pH 8.0 25% PEG Smear Low cryo: 20 % PEG 400