7YTJ

Cryo-EM structure of VTC complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Vacuolar transporter chaperone 4	polymer	754	86426.0	1	UniProt (P47075) Pfam (PF09359) Pfam (PF02656)	Saccharomyces cerevisiae (baker's yeast)	Phosphate metabolism protein 3
2	B, C, D (A, B, C)	Vacuolar transporter chaperone 1	polymer	143	15943.7	3	UniProt (P40046) Pfam (PF02656)	Saccharomyces cerevisiae (baker's yeast)	Negative regulator of CDC42 protein 1,Phosphate metabolism protein 4
3	E (E)	Vacuolar transporter chaperone 3	polymer	864	100120.8	1	UniProt (Q02725) Pfam (PF09359) Pfam (PF02656)	Saccharomyces cerevisiae (baker's yeast)	Phosphate metabolism protein 2
4	F, G, I (D)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	3	Chemie (IHP)
5	H, L (D, E)	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	2	Chemie (PC1)
6	J, K (D)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)

Sequence modifications

D: 3 - 722 (UniProt: P47075)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Ala 265	Arg 264	conflict
Ala 267	Arg 266	conflict
Asn 427	Glu 426	conflict
Leu 723	-	expression tag
Glu 724	-	expression tag
Ser 725	-	expression tag
Ala 726	-	expression tag
Trp 727	-	expression tag
Ser 728	-	expression tag
His 729	-	expression tag
Pro 730	-	expression tag
Gln 731	-	expression tag
Phe 732	-	expression tag
Glu 733	-	expression tag
Lys 734	-	expression tag
Gly 735	-	expression tag
Gly 736	-	expression tag
Gly 737	-	expression tag
Ser 738	-	expression tag
Gly 739	-	expression tag
Gly 740	-	expression tag
Gly 741	-	expression tag
Ser 742	-	expression tag
Gly 743	-	expression tag
Gly 744	-	expression tag
Ser 745	-	expression tag
Ala 746	-	expression tag
Trp 747	-	expression tag
Ser 748	-	expression tag
His 749	-	expression tag
Pro 750	-	expression tag
Gln 751	-	expression tag
Phe 752	-	expression tag
Glu 753	-	expression tag
Lys 754	-	expression tag

A, B, C: 36 - 163 (UniProt: P40046)

PDB	External Database	Details
Met 34	-	initiating methionine
Ala 35	-	expression tag
Leu 164	-	expression tag
Glu 165	-	expression tag
Ser 166	-	expression tag
Ala 167	-	expression tag
Tyr 168	-	expression tag
Pro 169	-	expression tag
Tyr 170	-	expression tag
Asp 171	-	expression tag
Val 172	-	expression tag
Pro 173	-	expression tag
Asp 174	-	expression tag
Tyr 175	-	expression tag
Ala 176	-	expression tag

E: 30 - 864 (UniProt: Q02725)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Tyr 3	-	expression tag
Lys 4	-	expression tag
Asp 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Lys 9	-	expression tag
Gly 10	-	expression tag
Asp 11	-	expression tag
Tyr 12	-	expression tag
Lys 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Asp 16	-	expression tag
Asp 17	-	expression tag
Lys 18	-	expression tag
Ile 19	-	expression tag
Asp 20	-	expression tag
Tyr 21	-	expression tag
Lys 22	-	expression tag
Asp 23	-	expression tag
Asp 24	-	expression tag
Asp 25	-	expression tag
Asp 26	-	expression tag
Lys 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	234377.9
Non-Polymers*	Number of molecules	7
	Total formula weight	3750.3
All*	Total formula weight	238128.2

*Water molecules are not included.