7YSH

Cryo-EM Structure of FGF23-FGFR1c-aKlotho-HS Quaternary Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Klotho	polymer	948	109164.8	1	UniProt (Q9UEF7) Pfam (PF00232)	Homo sapiens (human)
2	B (B)	Fibroblast growth factor 23	polymer	273	30367.0	1	UniProt (Q9GZV9) Pfam (PF00167)	Homo sapiens (human)	FGF-23,Phosphatonin,Tumor-derived hypophosphatemia-inducing factor
3	C, D (D, E)	Isoform 20 of Fibroblast growth factor receptor 1	polymer	234	26373.0	2	UniProt (P11362-20) Pfam (PF07679) UniProt (by SIFTS) (P11362)	Homo sapiens (human)	FGFR-1,Basic fibroblast growth factor receptor 1,BFGFR,bFGF-R-1,Fms-like tyrosine kinase 2,FLT-2,N-sam,Proto-oncogene c-Fgr,"c" splice isoform of FGFR1,FGFR1c
4	E (F)	2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose	branched		2649.2	1	In PDB GlyTouCan (G10608JX)
5	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	G (D)	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)

Sequence modifications

B: 25 - 251 (UniProt: Q9GZV9)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Met -4	-	expression tag
Lys -3	-	expression tag
Glu -2	-	expression tag
Thr -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
Ala 2	-	expression tag
Lys 3	-	expression tag
Phe 4	-	expression tag
Glu 5	-	expression tag
Arg 6	-	expression tag
Gln 7	-	expression tag
His 8	-	expression tag
Met 9	-	expression tag
Asp 10	-	expression tag
Ser 11	-	expression tag
Pro 12	-	expression tag
Asp 13	-	expression tag
Leu 14	-	expression tag
Gly 15	-	expression tag
Thr 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Lys 21	-	expression tag
Ala 22	-	expression tag
Met 23	-	expression tag
Gly 24	-	expression tag
Gln 176	Arg 176	variant
Gln 179	Arg 179	variant

D, E: 142 - 366 (UniProt: P11362-20)

PDB	External Database	Details
Gly 367	-	expression tag
Thr 368	-	expression tag
Lys 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192277.8
Branched	Number of molecules	1
	Total formula weight	2649.2
Non-Polymers*	Number of molecules	2
	Total formula weight	129.0
All*	Total formula weight	195055.9

*Water molecules are not included.