7YEH

Cryo-EM structure of human OGT-OGA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit	polymer	1052	117953.4	2	UniProt (O15294) Pfam (PF13414) Pfam (PF13844) Pfam (PF13181) Pfam (PF00515) Pfam (PF13424) In PDB	Homo sapiens (human)	O-GlcNAc transferase subunit p110,O-linked N-acetylglucosamine transferase 110 kDa subunit,OGT
2	C, D	Protein O-GlcNAcase	polymer	916	103020.9	2	UniProt (O60502) Pfam (PF07555) In PDB	Homo sapiens (human)	OGA,Beta-N-acetylglucosaminidase,Beta-N-acetylhexosaminidase,Beta-hexosaminidase,Meningioma-expressed antigen 5,N-acetyl-beta-D-glucosaminidase,N-acetyl-beta-glucosaminidase,Nuclear cytoplasmic O-GlcNAcase and acetyltransferase,NCOAT
3	A	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

B, A: 1 - 1046 (UniProt: O15294)

PDB	External Database	Details
Met 25	Ala 25	conflict
Glu 67	Gln 67	conflict
His 1047	-	expression tag
His 1048	-	expression tag
His 1049	-	expression tag
His 1050	-	expression tag
His 1051	-	expression tag
His 1052	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	441948.6
Non-Polymers*	Number of molecules	2
	Total formula weight	625.4
All*	Total formula weight	442574.0

*Water molecules are not included.