7Y38

Molecular architecture of the chikungunya virus replication complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	mRNA-capping enzyme nsP1,affinity-tag (strepII-3XFLAG)	polymer	573	64328.9	12	UniProt (Q8JUX6) Pfam (PF01660)	Chikungunya virus strain S27-African prototype	Non-structural protein 1
2	M (X)	RNA-directed RNA polymerase nsP4	polymer	611	68373.3	1	Pfam (PF00978) UniProt (by SIFTS) (O90368)	Onyong-nyong virus
3	N (Y)	Protease nsP2	polymer	798	89599.8	1	UniProt (Q8JUX6) Pfam (PF20896) Pfam (PF01443) Pfam (PF01707)	Chikungunya virus strain S27-African prototype	Non-structural protein 2,nsP2
4	O (Z)	RNA (5'-R(P*CP*CP*A)-3')	polymer	3	894.6	1		in vitro transcription vector pT7-TP(deltai)
5	BA, EA, HA, KA, NA... (E, F, G, H, I...)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
6	AA, DA, GA, IA, LA... (D, E, F, G, H...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	12	Chemie (ATP)
7	CA, FA, JA, MA, PA... (E, F, G, H, I...)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	12	Chemie (GTP)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 516 (UniProt: Q8JUX6)

PDB	External Database	Details
Ala 37	His 37	engineered mutation

A, B, C, D, E, F, G, H, I, J, K, L: 517 - 552 (PDB: 7Y38)
A, B, C, D, E, F, G, H, I, J, K, L: 553 - 570 (UniProt: Q8JUX6)

PDB	External Database	Details
Asn 571	-	expression tag
Gly 572	-	expression tag
Leu 573	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	930815.0
Non-Polymers*	Number of molecules	36
	Total formula weight	13149.2
All*	Total formula weight	943964.2

*Water molecules are not included.