7XWO

Neurokinin A bound to active human neurokinin 2 receptor in complex with G324

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Soluble cytochrome b562,Substance-K receptor	polymer	505	57108.2	1	UniProt (P0ABE7) UniProt (P21452) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562,SKR,NK-2 receptor,NK-2R,Neurokinin A receptor,Tachykinin receptor 2
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37784.3	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (E)	scFv	polymer	304	32692.5	1		Rattus norvegicus
4	D (F)	G324	polymer	361	41710.3	1		Homo sapiens
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
6	F (D)	HIS-LYS-THR-ASP-SER-PHE-VAL-GLY-LEU-MET-NH2	polymer	11	1207.4	1	UniProt (by SIFTS) (P20366)	Homo sapiens

Sequence modifications

B: -105 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -153	-	initiating methionine
Lys -152	-	expression tag
Thr -151	-	expression tag
Ile -150	-	expression tag
Ile -149	-	expression tag
Ala -148	-	expression tag
Leu -147	-	expression tag
Ser -146	-	expression tag
Tyr -145	-	expression tag
Ile -144	-	expression tag
Phe -143	-	expression tag
Cys -142	-	expression tag
Leu -141	-	expression tag
Val -140	-	expression tag
Phe -139	-	expression tag
Ala -138	-	expression tag
Asp -137	-	expression tag
Tyr -136	-	expression tag
Lys -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Asp -132	-	expression tag
Asp -131	-	expression tag
Ala -130	-	expression tag
Lys -129	-	expression tag
Leu -128	-	expression tag
Gln -127	-	expression tag
Thr -126	-	expression tag
Met -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
Glu -114	-	expression tag
Asn -113	-	expression tag
Leu -112	-	expression tag
Tyr -111	-	expression tag
Phe -110	-	expression tag
Gln -109	-	expression tag
Gly -108	-	expression tag
Gly -107	-	expression tag
Thr -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	-	linker

B: 1 - 345 (UniProt: P21452)

PDB	External Database	Details
Lys 346	-	expression tag
Leu 347	-	expression tag
Val 348	-	expression tag
Glu 349	-	expression tag
Lys 350	-	expression tag
Tyr 351	-	expression tag

C: 7 - 345 (UniProt: P54311)

PDB	External Database	Details
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178363.8
All*	Total formula weight	178363.8

*Water molecules are not included.