7XJI

Solabegron-activated dog beta3 adrenergic receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44255.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (N)	Nanobody-35	polymer	137	15015.7	1		unidentified
4	D (R)	Beta-3 adrenergic receptor	polymer	395	42441.3	1	UniProt (O02662) Pfam (PF00001)	Canis lupus familiaris (dog)	Beta-3 adrenoreceptor,Beta-3 adrenoceptor
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
6	F (R)	3-[3-[2-[[(2~{S})-2-(3-chlorophenyl)-2-oxidanyl-ethyl]amino]ethylamino]phenyl]benzoic acid	non-polymer		410.9	1	Chemie (EI5)

Sequence modifications

A: 5 - 384 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation
Tyr 63	Leu 63	engineered mutation
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	engineered mutation
Ile 365	Val 375	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 2 - 379 (UniProt: O02662)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Glu 380	-	expression tag
Asn 381	-	expression tag
Leu 382	-	expression tag
Tyr 383	-	expression tag
Phe 384	-	expression tag
Gln 385	-	expression tag
Gly 386	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148004.7
Non-Polymers*	Number of molecules	1
	Total formula weight	410.9
All*	Total formula weight	148415.6

*Water molecules are not included.