7XBX

Cryo-EM structure of the human chemokine receptor CX3CR1 in complex with CX3CL1 and Gi1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	D	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38245.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	E	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	R	Processed fractalkine,CX3C chemokine receptor 1	polymer	463	51558.3	1	UniProt (P78423) UniProt (P49238) Pfam (PF00048) Pfam (PF00001) In PDB	Homo sapiens (human)	C-X3-C CKR-1,CX3CR1,Beta chemokine receptor-like 1,CMK-BRL-1,CMK-BRL1,Fractalkine receptor,G-protein coupled receptor 13,V28
5	R	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

C: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

D: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

R: 1 - 76 (UniProt: P78423)

PDB	External Database	Details
Cys 35	Gly 59	engineered mutation
Ser 77	-	linker
Gly 78	-	linker
Ser 79	-	linker
Gly 80	-	linker
Ser 81	-	linker
Gly 82	-	linker
Ser 83	-	linker
Gly 84	-	linker
Ser 85	-	linker
Gly 86	-	linker
Ser 87	-	linker
Gly 88	-	linker
Ser 89	-	linker
Gly 90	-	linker
Ser 91	-	linker
Gly 92	-	linker
Ser 93	-	linker
Gly 94	-	linker
Ser 95	-	linker
Gly 96	-	linker
Ser 97	-	linker
Gly 98	-	linker
Ser 99	-	linker
Gly 100	-	linker
Ser 101	-	linker
Gly 102	-	linker
Ser 103	-	linker

R: 104 - 417 (UniProt: P49238)

PDB	External Database	Details
Leu 222	Ile 120	engineered mutation
Cys 278	Leu 176	engineered mutation
Ser 323	Cys 221	engineered mutation
Val 352	Met 250	engineered mutation
Glu 418	-	expression tag
Phe 419	-	expression tag
Leu 420	-	expression tag
Glu 421	-	expression tag
Val 422	-	expression tag
Leu 423	-	expression tag
Phe 424	-	expression tag
Gln 425	-	expression tag
Gly 426	-	expression tag
Pro 427	-	expression tag
Trp 428	-	expression tag
Ser 429	-	expression tag
His 430	-	expression tag
Pro 431	-	expression tag
Gln 432	-	expression tag
Phe 433	-	expression tag
Glu 434	-	expression tag
Lys 435	-	expression tag
Gly 436	-	expression tag
Gly 437	-	expression tag
Gly 438	-	expression tag
Ser 439	-	expression tag
Gly 440	-	expression tag
Gly 441	-	expression tag
Gly 442	-	expression tag
Ser 443	-	expression tag
Gly 444	-	expression tag
Gly 445	-	expression tag
Ser 446	-	expression tag
Ala 447	-	expression tag
Trp 448	-	expression tag
Ser 449	-	expression tag
His 450	-	expression tag
Pro 451	-	expression tag
Gln 452	-	expression tag
Phe 453	-	expression tag
Glu 454	-	expression tag
Lys 455	-	expression tag
Asp 456	-	expression tag
Tyr 457	-	expression tag
Lys 458	-	expression tag
Asp 459	-	expression tag
Asp 460	-	expression tag
Asp 461	-	expression tag
Asp 462	-	expression tag
Lys 463	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	138112.3
Non-Polymers*	Number of molecules	2
	Total formula weight	773.3
All*	Total formula weight	138885.6

*Water molecules are not included.