7WNM

Structure of SARS-CoV-2 Gamma variant receptor-binding domain complexed with high affinity human ACE2 mutant (T27F,R273Q)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Angiotensin-converting enzyme 2	polymer	740	85416.4	1	UniProt (Q9BYF1) Pfam (PF01401) Pfam (PF16959)	Homo sapiens (human)	Angiotensin-converting enzyme homolog,ACEH,Angiotensin-converting enzyme-related carboxypeptidase,ACE-related carboxypeptidase,Metalloprotease MPROT15
2	B (A)	Spike protein S1	polymer	244	27650.4	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2
3	C (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	E, F (B, A)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

B: 1 - 740 (UniProt: Q9BYF1)

PDB	External Database	Details
Phe 27	Thr 27	engineered mutation
Gln 273	Arg 273	engineered mutation

A: 319 - 541 (UniProt: P0DTC2)

PDB	External Database	Details
Met 304	-	expression tag
Phe 305	-	expression tag
Val 306	-	expression tag
Phe 307	-	expression tag
Leu 308	-	expression tag
Val 309	-	expression tag
Leu 310	-	expression tag
Leu 311	-	expression tag
Pro 312	-	expression tag
Leu 313	-	expression tag
Val 314	-	expression tag
Ser 315	-	expression tag
Ser 316	-	expression tag
Gln 317	-	expression tag
Cys 318	-	expression tag
Thr 417	Lys 417	variant
Lys 484	Glu 484	variant
Tyr 501	Asn 501	engineered mutation
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	113066.8
Non-Polymers*	Number of molecules	2
	Total formula weight	286.6
All*	Total formula weight	113353.4

*Water molecules are not included.