7WN6

Cryo-EM structure of VWF D'D3 dimer (R1136M/E1143M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, F (A, B, E, G)	von Willebrand antigen 2	polymer	741	81427.7	4	UniProt (P04275) Pfam (PF00094) Pfam (PF08742) Pfam (PF01826)	Homo sapiens (human)	von Willebrand antigen II
2	B, D, G, H (F, H, C, D)	von Willebrand factor	polymer	490	54208.9	4	UniProt (P04275) Pfam (PF16164) Pfam (PF00094) Pfam (PF08742) Pfam (PF01826)	Homo sapiens (human)	vWF
3	AA, BA, EA, FA, I... (E, G, A, C, F...)	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)
4	CA, DA, GA, HA, IA... (E, G, C, A, F...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	20	Chemie (NAG)

Sequence modifications

F, H, C, D: 764 - 1241 (UniProt: P04275)

PDB	External Database	Details
Met 1136	Arg 1136	engineered mutation
Met 1143	Glu 1143	engineered mutation
Gly 1242	-	expression tag
Leu 1243	-	expression tag
Val 1244	-	expression tag
Val 1245	-	expression tag
Pro 1246	-	expression tag
Pro 1247	-	expression tag
His 1248	-	expression tag
His 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	542546.4
Non-Polymers*	Number of molecules	36
	Total formula weight	5065.4
All*	Total formula weight	547611.8

*Water molecules are not included.