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- EMDB-32621: Cryo-EM structure of VWF D'D3 dimer (wild type) complexed with D1... -

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Basic information

Entry
Database: EMDB / ID: EMD-32621
TitleCryo-EM structure of VWF D'D3 dimer (wild type) complexed with D1D2 at 3.4 angstron resolution (1 unit)
Map data
Sample
  • Complex: VWF D'D3-D1D2 complex (1 unit)
    • Protein or peptide: von Willebrand antigen 2
    • Protein or peptide: von Willebrand factor
  • Ligand: CALCIUM ION
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Keywordsblood / VWF / von Willebrand factor / von Willebrand disease / blood coagulation / blood clotting / multimer assembly / VWF assembly / D'D3 domain / D1D2 domain / D'D3 dimer / D1D2 Dimer / VWF Tube / repeating unit
Function / homology
Function and homology information


Defective VWF binding to collagen type I / Enhanced cleavage of VWF variant by ADAMTS13 / Defective VWF cleavage by ADAMTS13 variant / Defective F8 binding to von Willebrand factor / Enhanced binding of GP1BA variant to VWF multimer:collagen / Defective binding of VWF variant to GPIb:IX:V / Weibel-Palade body / hemostasis / platelet alpha granule / Platelet Adhesion to exposed collagen ...Defective VWF binding to collagen type I / Enhanced cleavage of VWF variant by ADAMTS13 / Defective VWF cleavage by ADAMTS13 variant / Defective F8 binding to von Willebrand factor / Enhanced binding of GP1BA variant to VWF multimer:collagen / Defective binding of VWF variant to GPIb:IX:V / Weibel-Palade body / hemostasis / platelet alpha granule / Platelet Adhesion to exposed collagen / positive regulation of intracellular signal transduction / GP1b-IX-V activation signalling / p130Cas linkage to MAPK signaling for integrins / cell-substrate adhesion / Defective F8 cleavage by thrombin / Platelet Aggregation (Plug Formation) / immunoglobulin binding / GRB2:SOS provides linkage to MAPK signaling for Integrins / Integrin cell surface interactions / collagen binding / Intrinsic Pathway of Fibrin Clot Formation / Integrin signaling / extracellular matrix / platelet alpha granule lumen / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / platelet activation / response to wounding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Signaling by BRAF and RAF1 fusions / blood coagulation / integrin binding / Platelet degranulation / protein-folding chaperone binding / protease binding / collagen-containing extracellular matrix / cell adhesion / endoplasmic reticulum / extracellular space / extracellular exosome / extracellular region / identical protein binding
Similarity search - Function
von Willebrand factor, VWA N-terminal domain / Von Willebrand factor / VWA N-terminal / : / C8 domain / Uncharacterised domain, cysteine-rich / C8 / von Willebrand factor, type D domain / von Willebrand factor type D domain / VWFD domain profile. ...von Willebrand factor, VWA N-terminal domain / Von Willebrand factor / VWA N-terminal / : / C8 domain / Uncharacterised domain, cysteine-rich / C8 / von Willebrand factor, type D domain / von Willebrand factor type D domain / VWFD domain profile. / von Willebrand factor (vWF) type D domain / von Willebrand factor (vWF) type C domain / Trypsin Inhibitor-like, cysteine rich domain / Serine protease inhibitor-like superfamily / Trypsin Inhibitor like cysteine rich domain / C-terminal cystine knot signature. / C-terminal cystine knot domain profile. / Cystine knot, C-terminal / C-terminal cystine knot-like domain (CTCK) / von Willebrand factor type C domain / VWFC domain signature. / VWFC domain profile. / von Willebrand factor (vWF) type C domain / VWFC domain / von Willebrand factor type A domain / von Willebrand factor (vWF) type A domain / VWFA domain profile. / von Willebrand factor, type A / von Willebrand factor A-like domain superfamily
Similarity search - Domain/homology
von Willebrand factor
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsZeng JW / Shu ZM / Zhou AW
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2016YFA0501103 China
CitationJournal: Cell Discov / Year: 2022
Title: Structural mechanism of VWF D'D3 dimer formation.
Authors: Zimei Shu / Jianwei Zeng / Li Xia / Haiyan Cai / Aiwu Zhou /
History
DepositionJan 17, 2022-
Header (metadata) releaseMay 18, 2022-
Map releaseMay 18, 2022-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32621.png

Downloads & links

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Map

FileDownload / File: emd_32621.map.gz / Format: CCP4 / Size: 22.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.97 Å/pix.
x 169 pix.
= 163.93 Å		0.97 Å/pix.
x 185 pix.
= 179.45 Å		0.97 Å/pix.
x 190 pix.
= 184.3 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 0.97 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.009
Minimum - Maximum-0.037703242 - 0.07030591
Average (Standard dev.)0.0005323077 (±0.0037780441)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions185190169
Spacing190185169
CellA: 184.3 Å / B: 179.45001 Å / C: 163.93001 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : VWF D'D3-D1D2 complex (1 unit)

EntireName: VWF D'D3-D1D2 complex (1 unit)
Components
  • Complex: VWF D'D3-D1D2 complex (1 unit)
    • Protein or peptide: von Willebrand antigen 2
    • Protein or peptide: von Willebrand factor
  • Ligand: CALCIUM ION
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: VWF D'D3-D1D2 complex (1 unit)

SupramoleculeName: VWF D'D3-D1D2 complex (1 unit) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: von Willebrand antigen 2

MacromoleculeName: von Willebrand antigen 2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 81.427703 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AEGTRGRSST ARCSLFGSDF VNTFDGSMYS FAGYCSYLLA GGCQKRSFSI IGDFQNGKRV SLSVYLGEFF DIHLFVNGTV TQGDQRVSM PYASKGLYLE TEAGYYKLSG EAYGFVARID GSGNFQVLLS DRYFNKTCGL CGNFNIFAED DFMTQEGTLT S DPYDFANS ...String:
AEGTRGRSST ARCSLFGSDF VNTFDGSMYS FAGYCSYLLA GGCQKRSFSI IGDFQNGKRV SLSVYLGEFF DIHLFVNGTV TQGDQRVSM PYASKGLYLE TEAGYYKLSG EAYGFVARID GSGNFQVLLS DRYFNKTCGL CGNFNIFAED DFMTQEGTLT S DPYDFANS WALSSGEQWC ERASPPSSSC NISSGEMQKG LWEQCQLLKS TSVFARCHPL VDPEPFVALC EKTLCECAGG LE CACPALL EYARTCAQEG MVLYGWTDHS ACSPVCPAGM EYRQCVSPCA RTCQSLHINE MCQERCVDGC SCPEGQLLDE GLC VESTEC PCVHSGKRYP PGTSLSRDCN TCICRNSQWI CSNEECPGEC LVTGQSHFKS FDNRYFTFSG ICQYLLARDC QDHS FSIVI ETVQCADDRD AVCTRSVTVR LPGLHNSLVK LKHGAGVAMD GQDVQLPLLK GDLRIQHTVT ASVRLSYGED LQMDW DGRG RLLVKLSPVY AGKTCGLCGN YNGNQGDDFL TPSGLAEPRV EDFGNAWKLH GDCQDLQKQH SDPCALNPRM TRFSEE ACA VLTSPTFEAC HRAVSPLPYL RNCRYDVCSC SDGRECLCGA LASYAAACAG RGVRVAWREP GRCELNCPKG QVYLQCG TP CNLTCRSLSY PDEECNEACL EGCFCPPGLY MDERGDCVPK AQCPCYYDGE IFQPEDIFSD HHTMCYCEDG FMHCTMSG V PGSLLPDAVL SSPLSHRSKR

UniProtKB: von Willebrand factor

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Macromolecule #2: von Willebrand factor

MacromoleculeName: von Willebrand factor / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 54.232809 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SLSCRPPMVK LVCPADNLRA EGLECTKTCQ NYDLECMSMG CVSGCLCPPG MVRHENRCVA LERCPCFHQG KEYAPGETVK IGCNTCVCQ DRKWNCTDHV CDATCSTIGM AHYLTFDGLK YLFPGECQYV LVQDYCGSNP GTFRILVGNK GCSHPSVKCK K RVTILVEG ...String:
SLSCRPPMVK LVCPADNLRA EGLECTKTCQ NYDLECMSMG CVSGCLCPPG MVRHENRCVA LERCPCFHQG KEYAPGETVK IGCNTCVCQ DRKWNCTDHV CDATCSTIGM AHYLTFDGLK YLFPGECQYV LVQDYCGSNP GTFRILVGNK GCSHPSVKCK K RVTILVEG GEIELFDGEV NVKRPMKDET HFEVVESGRY IILLLGKALS VVWDRHLSIS VVLKQTYQEK VCGLCGNFDG IQ NNDLTSS NLQVEEDPVD FGNSWKVSSQ CADTRKVPLD SSPATCHNNI MKQTMVDSSC RILTSDVFQD CNKLVDPEPY LDV CIYDTC SCESIGDCAC FCDTIAAYAH VCAQHGKVVT WRTATLCPQS CEERNLRENG YECEWRYNSC APACQVTCQH PEPL ACPVQ CVEGCHAHCP PGKILDELLQ TCVDPEDCPV CEVAGRRFAS GKKVTLNPSD PEHCQICHCD VVNLTCEACQ EPGGL VVPP HHHHHH

UniProtKB: von Willebrand factor

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Macromolecule #3: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 3 / Number of copies: 6 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 8 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 154669
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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