7W6A

Crystal structure of the MLL1 (N3861I/Q3867L/C3882SS)-RBBP5-ASH2L complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Set1/Ash2 histone methyltransferase complex subunit ASH2	polymer	184	20597.4	1	UniProt (Q9UBL3) Pfam (PF00622) In PDB	Homo sapiens (human)	ASH2-like protein
2	C	Histone-lysine N-methyltransferase 2A	polymer	159	18314.2	1	UniProt (Q03164) Pfam (PF00856) In PDB	Homo sapiens (human)
3	F	Retinoblastoma-binding protein 5	polymer	27	3255.3	1	UniProt (Q15291) In PDB	Homo sapiens (human)	RBBP-5,Retinoblastoma-binding protein RBQ-3
4	C	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
5	C	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6		water	water		18.0	116	Chemie (HOH)

Sequence modifications

A: 286 - 438 (UniProt: Q9UBL3)

PDB	External Database	Details
Ser 285	-	expression tag

A: 445 - 504 (UniProt: Q9UBL3)

PDB	External Database	Details
Ile 439	-	linker
Ser 440	-	linker
Gly 441	-	linker
Arg 442	-	linker
Gly 443	-	linker
Ser 444	-	linker

C: 3813 - 3970 (UniProt: Q03164)

PDB	External Database	Details
Ser 3812	-	expression tag
Ile 3861	Asn 3861	engineered mutation
Leu 3867	Gln 3867	engineered mutation
Ser 3882	-	insertion
Ser 3883	Cys 3882	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	42166.9
Non-Polymers*	Number of molecules	2
	Total formula weight	449.8
All*	Total formula weight	42616.7

*Water molecules are not included.