7VW3
Cryo-EM structure of SaCas9-sgRNA-DNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1052 | 123940.5 | 1 | UniProt (J7RUA5) Pfam (PF13395) Pfam (PF22702) Pfam (PF18061) Pfam (PF18070) Pfam (PF21574) | Staphylococcus aureus | SaCas9 |
| 2 | B (B) | single-guide RNA | polymer | 104 | 33217.5 | 1 | synthetic construct | sgRNA | |
| 3 | C (C) | Target DNA strand | polymer | 40 | 12372.0 | 1 | synthetic construct | TS | |
| 4 | D (D) | Non-target DNA strand | polymer | 28 | 8582.5 | 1 | synthetic construct | NTS | |
| 5 | E, F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
A: 2 - 1053 (UniProt: J7RUA5)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 580 | Asn 580 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 178112.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 178185.5 |
