7VPX
The cryo-EM structure of the human pre-A complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Splicing factor 3A subunit 2 | polymer | 464 | 49327.4 | 1 | UniProt (Q15428) Pfam (PF12874) Pfam (PF16835) | Homo sapiens (human) | SF3a66,Spliceosome-associated protein 62,SAP 62 |
| 2 | B (D) | Splicing factor 1 | polymer | 639 | 68401.7 | 1 | UniProt (Q15637) Pfam (PF16275) Pfam (PF22675) Pfam (PF00098) | Homo sapiens (human) | Mammalian branch point-binding protein,BBP,mBBP,Transcription factor ZFM1,Zinc finger gene in MEN1 locus,Zinc finger protein 162 |
| 3 | C (J) | DnaJ homolog subfamily C member 8 | polymer | 253 | 29900.8 | 1 | UniProt (O75937) Pfam (PF00226) | Homo sapiens (human) | |
| 4 | D (H) | U2 snRNA | polymer | 188 | 60186.4 | 1 | GenBank (340097) | Homo sapiens (human) | |
| 5 | E (1) | Splicing factor 3B subunit 1 | polymer | 1304 | 146024.9 | 1 | UniProt (O75533) Pfam (PF08920) Pfam (PF22646) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 6 | F (2) | Splicing factor 3B subunit 2 | polymer | 895 | 100377.8 | 1 | UniProt (Q13435) Pfam (PF02037) Pfam (PF04037) Pfam (PF04046) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 145 kDa subunit,SF3b145,Spliceosome-associated protein 145,SAP 145 |
| 7 | G (3) | Splicing factor 3B subunit 3 | polymer | 1217 | 135718.8 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 8 | H (4) | Splicing factor 3B subunit 4 | polymer | 424 | 44436.6 | 1 | UniProt (Q15427) Pfam (PF00076) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 49 kDa subunit,Spliceosome-associated protein 49,SAP 49 |
| 9 | I (5) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) | Homo sapiens (human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 10 | J (A) | Splicing factor 3A subunit 1 | polymer | 793 | 88991.1 | 1 | UniProt (Q15459) Pfam (PF01805) Pfam (PF12230) Pfam (PF00240) | Homo sapiens (human) | SF3a120,Spliceosome-associated protein 114,SAP 114 |
| 11 | K (C) | Splicing factor 3A subunit 3 | polymer | 501 | 58934.8 | 1 | UniProt (Q12874) Pfam (PF12108) Pfam (PF16837) Pfam (PF13297) Pfam (PF11931) | Homo sapiens (human) | SF3a60,Spliceosome-associated protein 61,SAP 61 |
| 12 | L (E) | Probable ATP-dependent RNA helicase DDX46 | polymer | 1031 | 117576.5 | 1 | UniProt (Q7L014) Pfam (PF00270) Pfam (PF00271) | Homo sapiens (human) | DEAD box protein 46,PRP5 homolog |
| 13 | M (F) | U2 small nuclear ribonucleoprotein A' | polymer | 255 | 28456.6 | 1 | UniProt (P09661) Pfam (PF14580) | Homo sapiens (human) | U2 snRNP A' |
| 14 | N (G) | U2 small nuclear ribonucleoprotein B'' | polymer | 225 | 25524.4 | 1 | UniProt (P08579) Pfam (PF00076) | Homo sapiens (human) | U2 snRNP B'' |
| 15 | FA, O (a, i) | Small nuclear ribonucleoprotein Sm D2 | polymer | 118 | 13551.9 | 2 | UniProt (P62316) Pfam (PF01423) | Homo sapiens (human) | Sm-D2,snRNP core protein D2 |
| 16 | P, Z (b, m) | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 2 | UniProt (P62306) Pfam (PF01423) | Homo sapiens (human) | snRNP-F,Sm protein F,SmF |
| 17 | EA, Q (c, l) | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 2 | UniProt (P62304) Pfam (PF01423) | Homo sapiens (human) | snRNP-E,Sm protein E,SmE |
| 18 | DA, R (d, n) | Small nuclear ribonucleoprotein G | polymer | 76 | 8508.1 | 2 | UniProt (P62308) Pfam (PF01423) | Homo sapiens (human) | snRNP-G,Sm protein G,SmG |
| 19 | HA, S (e, j) | Small nuclear ribonucleoprotein Sm D3 | polymer | 126 | 13940.3 | 2 | UniProt (P62318) Pfam (PF01423) | Homo sapiens (human) | Sm-D3,snRNP core protein D3 |
| 20 | BA, T (f, k) | Small nuclear ribonucleoprotein-associated proteins B and B' | polymer | 240 | 24642.1 | 2 | UniProt (P14678) Pfam (PF01423) | Homo sapiens (human) | snRNP-B,Sm protein B/B',SmB/B' |
| 21 | AA, U (g, h) | Small nuclear ribonucleoprotein Sm D1 | polymer | 119 | 13310.7 | 2 | UniProt (P62314) Pfam (PF01423) | Homo sapiens (human) | Sm-D1,Sm-D autoantigen,snRNP core protein D1 |
| 22 | V (I) | pre-mRNA | polymer | 259 | 83090.0 | 1 | unidentified adenovirus | ||
| 23 | W (K) | 5SS | polymer | 10 | 3257.0 | 1 | unidentified adenovirus | ||
| 24 | X (L) | U1 snRNA | polymer | 164 | 52697.1 | 1 | GenBank (1781322691) | Homo sapiens (human) | |
| 25 | Y (O) | U1 small nuclear ribonucleoprotein 70 kDa | polymer | 437 | 51683.2 | 1 | UniProt (P08621) Pfam (PF12220) Pfam (PF00076) | Homo sapiens (human) | |
| 26 | CA (M) | U1 small nuclear ribonucleoprotein A | polymer | 282 | 31319.5 | 1 | UniProt (P09012) Pfam (PF00076) | Homo sapiens (human) | |
| 27 | GA (N) | U1 small nuclear ribonucleoprotein C | polymer | 159 | 17415.7 | 1 | UniProt (P09234) Pfam (PF06220) | Homo sapiens (human) | U1 snRNP C,U1-C,U1C |
| 28 | IA (6) | PHD finger-like domain-containing protein 5A | polymer | 110 | 12427.5 | 1 | UniProt (Q7RTV0) Pfam (PF03660) | Homo sapiens (human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 29 | JA, KA, LA, MA, NA... (B, C, N, 6) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 30 | PA (6) | spliceostatin A (form II) | non-polymer | 523.7 | 1 | Chemie (SJT) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1404907.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 916.1 | |
| All* | Total formula weight | 1405823.1 |
