7UX2
cryo-EM structure of the Raptor-TFEB-Rag-Ragulator complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Regulatory-associated protein of mTOR | polymer | 1335 | 149200.0 | 1 | UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) | Homo sapiens (human) | Raptor,p150 target of rapamycin (TOR)-scaffold protein |
| 2 | B, I (B, I) | Ras-related GTP-binding protein A | polymer | 313 | 36600.2 | 2 | UniProt (Q7L523) Pfam (PF04670) | Homo sapiens (human) | Rag A,RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1 |
| 3 | C, J (C, J) | Ras-related GTP-binding protein C | polymer | 399 | 44298.9 | 2 | UniProt (Q9HB90) Pfam (PF04670) | Homo sapiens (human) | RagC,GTPase-interacting protein 2,TIB929 |
| 4 | D, K (D, K) | Ragulator complex protein LAMTOR1 | polymer | 161 | 17762.8 | 2 | UniProt (Q6IAA8) Pfam (PF15454) | Homo sapiens (human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho |
| 5 | E, L (E, L) | Ragulator complex protein LAMTOR2 | polymer | 125 | 13517.5 | 2 | UniProt (Q9Y2Q5) Pfam (PF03259) | Homo sapiens (human) | Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3 |
| 6 | F, M (F, M) | Ragulator complex protein LAMTOR3 | polymer | 124 | 13637.7 | 2 | UniProt (Q9UHA4) Pfam (PF08923) | Homo sapiens (human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1 |
| 7 | G, N (G, N) | Ragulator complex protein LAMTOR4 | polymer | 99 | 10753.2 | 2 | UniProt (Q0VGL1) | Homo sapiens (human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 |
| 8 | H, O (H, O) | Ragulator complex protein LAMTOR5 | polymer | 91 | 9622.9 | 2 | UniProt (O43504) Pfam (PF16672) | Homo sapiens (human) | Hepatitis B virus X-interacting protein,HBX-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5 |
| 9 | P (P) | Transcription factor EB | polymer | 476 | 52926.6 | 1 | UniProt (P19484) Pfam (PF15951) Pfam (PF00010) Pfam (PF11851) | Homo sapiens (human) | Class E basic helix-loop-helix protein 35,bHLHe35 |
| 10 | Q, T (B, I) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 11 | R, U (B, I) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 12 | S, V (C, J) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) |
Sequence modifications
B, I: 1 - 313 (UniProt: Q7L523)
C, J: 1 - 399 (UniProt: Q9HB90)
| PDB | External Database | Details |
|---|---|---|
| Leu 66 | Gln 66 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Ser 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 494512.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1981.4 | |
| All* | Total formula weight | 496494.2 |
