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Cryo-EM structure of D-site Rac1-bound WAVE Regulatory Complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cytoplasmic FMR1-interacting protein 1 | polymer | 1253 | 145363.8 | 1 | UniProt (Q7L576) Pfam (PF07159) Pfam (PF05994) | Homo sapiens (human) | Specifically Rac1-associated protein 1,Sra-1,p140sra-1 |
| 2 | B (B) | Nck-associated protein 1 | polymer | 1128 | 128940.7 | 1 | UniProt (Q9Y2A7) Pfam (PF09735) | Homo sapiens (human) | NAP 1,Membrane-associated protein HEM-2,p125Nap1 |
| 3 | C (C) | Wiskott-Aldrich syndrome protein family member 1 | polymer | 323 | 37009.4 | 1 | UniProt (Q92558) Pfam (PF02205) | Homo sapiens (human) | WASP family protein member 1,Protein WAVE-1,Verprolin homology domain-containing protein 1 |
| 4 | D (D) | Protein BRICK1 | polymer | 75 | 8756.9 | 1 | UniProt (Q8WUW1) | Homo sapiens (human) | BRK1 |
| 5 | E (E) | Abl interactor 2 | polymer | 158 | 18041.5 | 1 | UniProt (E9PEZ7) Pfam (PF07815) UniProt (by SIFTS) (Q9NYB9) | Homo sapiens (human) | |
| 6 | F (F) | Ras-related C3 botulinum toxin substrate 1 | polymer | 188 | 21010.5 | 1 | UniProt (P63000) Pfam (PF00071) | Homo sapiens (human) | Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1 |
| 7 | G (F) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 8 | H (F) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) |
Sequence modifications
C: 1 - 466 (UniProt: Q92558)
C: 485 - 559 (UniProt: Q92558)
F: 1 - 188 (UniProt: P63000)
C: 485 - 559 (UniProt: Q92558)
| PDB | External Database | Details |
|---|---|---|
| Gly 467 | - | linker |
| Gly 468 | - | linker |
| Ser 469 | - | linker |
| Gly 470 | - | linker |
| Gly 471 | - | linker |
| Ser 472 | - | linker |
| Gly 473 | - | linker |
| Gly 474 | - | linker |
| Ser 475 | - | linker |
| Gly 476 | - | linker |
| Gly 477 | - | linker |
| Ser 478 | - | linker |
| Gly 479 | - | linker |
| Gly 480 | - | linker |
| Ser 481 | - | linker |
| Gly 482 | - | linker |
| Gly 483 | - | linker |
| Ser 484 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ser 29 | Pro 29 | engineered mutation |
| Leu 61 | Gln 61 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 359122.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 359670.3 |
