7UL4

CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mu-type opioid receptor	polymer	426	47920.7	1	UniProt (P42866) Pfam (PF00001) In PDB	Mus musculus (house mouse)	M-OR-1,MOR-1
2	D	Megabody 6	polymer	516	55662.1	1		synthetic construct
3	A	N-[(2S)-2-{[(3R,4R)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine	non-polymer		424.5	1	Chemie (NG0)
4		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 6 - 398 (UniProt: P42866)

PDB	External Database	Details
Met -19	-	initiating methionine
Lys -18	-	expression tag
Thr -17	-	expression tag
Ile -16	-	expression tag
Ile -15	-	expression tag
Ala -14	-	expression tag
Leu -13	-	expression tag
Ser -12	-	expression tag
Tyr -11	-	expression tag
Ile -10	-	expression tag
Phe -9	-	expression tag
Cys -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Phe -5	-	expression tag
Ala -4	-	expression tag
Asp -3	-	expression tag
Tyr -2	-	expression tag
Lys -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Ala 4	-	expression tag
Met 5	-	expression tag
Leu 264	Met 264	engineered mutation
Arg 269	Lys 269	engineered mutation
Asp 399	-	expression tag
Ile 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	103582.8
Non-Polymers*	Number of molecules	1
	Total formula weight	424.5
All*	Total formula weight	104007.3

*Water molecules are not included.