7UD6
Designed Enzyme SH3-588 (Catechol O-methyltransferase catalytic domain and Src homology 3 binding domain fusion)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Tyrosine-protein kinase Fyn,Catechol O-methyltransferase | polymer | 293 | 32634.0 | 1 | UniProt (E5RFS5) UniProt (P22734) Pfam (PF00018) Pfam (PF01596) UniProt (by SIFTS) (P06241) In PDB | Homo sapiens (human) | |
2 | A | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
3 | A | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
4 | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A: 2 - 57 (UniProt: E5RFS5)
A: 66 - 277 (UniProt: P22734)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Val 26 | Gln 110 | engineered mutation |
Arg 29 | Asn 113 | engineered mutation |
Leu 37 | Glu 121 | engineered mutation |
Val 39 | Arg 123 | engineered mutation |
Gln 46 | Thr 130 | engineered mutation |
Gly 58 | - | linker |
Gly 59 | - | linker |
Ser 60 | - | linker |
Gly 61 | - | linker |
Gly 62 | - | linker |
Ser 63 | - | linker |
Gly 64 | - | linker |
Gly 65 | - | linker |
PDB | External Database | Details |
---|---|---|
Glu 73 | Arg 54 | engineered mutation |
Phe 74 | Tyr 55 | engineered mutation |
Leu 77 | Gln 58 | engineered mutation |
Val 78 | Asn 59 | engineered mutation |
Gln 93 | Thr 74 | engineered mutation |
Tyr 97 | Gln 78 | engineered mutation |
Ala 98 | Lys 79 | engineered mutation |
Ile 153 | Met 134 | engineered mutation |
Cys 157 | Tyr 138 | engineered mutation |
Gly 278 | - | expression tag |
Glu 279 | - | expression tag |
Asn 280 | - | expression tag |
Leu 281 | - | expression tag |
Tyr 282 | - | expression tag |
Phe 283 | - | expression tag |
Gln 284 | - | expression tag |
Ser 285 | - | expression tag |
Ala 286 | - | expression tag |
Gly 287 | - | expression tag |
His 288 | - | expression tag |
His 289 | - | expression tag |
His 290 | - | expression tag |
His 291 | - | expression tag |
His 292 | - | expression tag |
His 293 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 32634.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 423.5 | |
All* | Total formula weight | 33057.5 |