7TX7

Cryo-EM structure of the human reduced folate carrier

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reduced folate transporter,Soluble cytochrome b562	polymer	678	74797.2	1	UniProt (P41440) UniProt (P0ABE7) Pfam (PF01770)	Homo sapiens (human)	FOLT,Intestinal folate carrier 1,IFC-1,Placental folate transporter,Reduced folate carrier protein,RFC,hRFC,Reduced folate transporter 1,RFT-1,Solute carrier family 19 member 1,hSLC19A1,Cytochrome b-562
2	B, C, D, E (A)	Digitonin	non-polymer		1229.3	4	Chemie (AJP)

Sequence modifications

A: 1 - 210 (UniProt: P41440)
A: 210 - 214 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 210	Met 29	engineered mutation
Ile 214	His 124	engineered mutation
Leu 214	-	linker

A: 214 - 591 (UniProt: P41440)

PDB	External Database	Details
Ala 592	-	expression tag
Asn 593	-	expression tag
Ser 594	-	expression tag
Leu 595	-	expression tag
Glu 596	-	expression tag
Val 597	-	expression tag
Leu 598	-	expression tag
Phe 599	-	expression tag
Gln 600	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74797.2
Non-Polymers*	Number of molecules	4
	Total formula weight	4917.2
All*	Total formula weight	79714.4

*Water molecules are not included.