7TX7

Cryo-EM structure of the human reduced folate carrier

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005506	molecular_function	iron ion binding
A	0005886	cellular_component	plasma membrane
A	0008518	molecular_function	folate:monoatomic anion antiporter activity
A	0009055	molecular_function	electron transfer activity
A	0015350	molecular_function	methotrexate transmembrane transporter activity
A	0015884	biological_process	folic acid transport
A	0016020	cellular_component	membrane
A	0020037	molecular_function	heme binding
A	0022900	biological_process	electron transport chain
A	0042597	cellular_component	periplasmic space
A	0046872	molecular_function	metal ion binding
A	0051180	biological_process	vitamin transport
A	0051958	biological_process	methotrexate transport
A	0090482	molecular_function	vitamin transmembrane transporter activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	43
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	34
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	18
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7TX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7TX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XPZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DEP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GOF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HII","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	12
Details	Region: {"description":"Required for substrate-binding","evidences":[{"source":"PubMed","id":"16115875","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36071163","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36575193","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GOE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HIJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36265513","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XQ2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9767079","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---