7TV4

Crystal structure of NEMO CoZi in complex with HOIP NZF1 and linear diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (B, D)	NF-kappa-B essential modulator	polymer	98	11293.8	2	UniProt (Q9Y6K9) Pfam (PF16516)	Homo sapiens (human)	NEMO,FIP-3,IkB kinase-associated protein 1,IKKAP1,Inhibitor of nuclear factor kappa-B kinase subunit gamma,I-kappa-B kinase subunit gamma,IKK-gamma,IKKG,IkB kinase subunit gamma,NF-kappa-B essential modifier
2	B, D (C, G)	Polyubiquitin-C	polymer	156	17423.9	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
3	E (K)	E3 ubiquitin-protein ligase RNF31	polymer	35	3728.3	1	UniProt (Q96EP0)	Homo sapiens (human)	HOIL-1-interacting protein,HOIP,RING finger protein 31,RING-type E3 ubiquitin transferase RNF31,Zinc in-between-RING-finger ubiquitin-associated domain protein
4	F (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	G, H, I, J (B, C, G, K)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

B, D: 257 - 346 (UniProt: Q9Y6K9)

PDB	External Database	Details
Gly 249	-	expression tag
Pro 250	-	expression tag
Leu 251	-	expression tag
Gly 252	-	expression tag
Ser 253	-	expression tag
Pro 254	-	expression tag
Glu 255	-	expression tag
Phe 256	-	expression tag

C, G: 1 - 152 (UniProt: P0CG48)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

K: 350 - 379 (UniProt: Q96EP0)

PDB	External Database	Details
Gly 345	-	expression tag
Pro 346	-	expression tag
Leu 347	-	expression tag
Gly 348	-	expression tag
Ser 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	61163.8
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	61229.2

*Water molecules are not included.