7TRA

Cascade complex from type I-A CRISPR-Cas system

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated helicase Cas3	polymer	572	61412.6	1	UniProt (A0A5C0XNV5)	Pyrococcus furiosus DSM 3638
2	B (B)	Cas8a	polymer	341	38800.7	1	UniProt (Q8U338) Pfam (PF09703)	Pyrococcus furiosus DSM 3638
3	C, D, N, O, P (C, D, E, F, G)	Cas11a	polymer	108	12208.9	5	UniProt (Q8U332)	Pyrococcus furiosus DSM 3638
4	E, F, G, H, Q... (H, I, J, K, L...)	Cas7a	polymer	336	36989.1	7	UniProt (Q8U333) Pfam (PF01905)	Pyrococcus furiosus DSM 3638
5	I (O)	Cas5a	polymer	256	29147.2	1	UniProt (A0A5C0XNV9)	Pyrococcus furiosus DSM 3638
6	J (Q)	CRISPR-associated endonuclease Cas3-HD	polymer	219	24778.2	1	UniProt (Q8U336) Pfam (PF18019)	Pyrococcus furiosus DSM 3638	CRISPR-associated ss-nucleic acid exo- and endonuclease Cas3-HD
7	K (R)	crRNA (44-MER)	polymer	44	14028.3	1		Escherichia coli
8	L (S)	Target strand DNA (44-MER)	polymer	44	13692.8	1		Escherichia coli
9	M (T)	Non-Target strand DNA (25-MER)	polymer	25	7727.1	1		Escherichia coli
10	T, U (Q)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)

Sequence modifications

A: 9 - 516 (UniProt: A0A5C0XNV5)

PDB	External Database	Details
Lys 228	Arg 228	conflict
-	Val 364	deletion
Ala 364	Glu 365	conflict
Ala 365	Asn 366	conflict
Ala 366	Cys 367	conflict
Ala 367	Ile 368	conflict
Ala 368	Glu 369	conflict
Ala 369	His 370	conflict
Ala 370	Glu 371	conflict
Ala 371	Lys 372	conflict
Ala 372	Ile 373	conflict
Ala 373	Val 374	conflict
Ala 374	Arg 375	conflict
Ala 375	Gly 376	conflict
Ala 376	Leu 377	conflict
Ala 377	Ser 378	conflict
Ala 378	Glu 379	conflict
Ala 379	Leu 380	conflict
Ala 380	Met 381	conflict
Ala 381	Glu 382	conflict
Ala 382	Lys 383	conflict
Ala 383	Ile 384	conflict
Ala 384	Gly 385	conflict
Ala 385	Glu 386	conflict
Ala 386	Asp 387	conflict
Ala 387	Thr 388	conflict
Ala 388	Val 389	conflict
Ala 389	Val 390	conflict
Ala 390	Phe 391	conflict
Val 392	Thr 393	conflict
Pro 451	-	insertion
-	Ala 499	deletion
Lys 517	-	expression tag
Ala 518	-	expression tag
Ala 519	-	expression tag
Ala 520	-	expression tag
Ala 521	-	expression tag
Ala 522	-	expression tag
Ala 523	-	expression tag
Ala 524	-	expression tag
Ala 525	-	expression tag
Ala 526	-	expression tag
Ala 527	-	expression tag
Ala 528	-	expression tag
Ala 529	-	expression tag
Ala 530	-	expression tag
Ala 531	-	expression tag
Ala 580	-	expression tag
Ala 581	-	expression tag
Ala 582	-	expression tag
Ala 583	-	expression tag
Ala 584	-	expression tag
Ala 585	-	expression tag
Ala 586	-	expression tag
Ala 587	-	expression tag
Ala 588	-	expression tag
Ala 589	-	expression tag
Ala 590	-	expression tag
Ala 591	-	expression tag
Ala 592	-	expression tag
Ala 593	-	expression tag
Ala 594	-	expression tag
Ala 595	-	expression tag
Ala 596	-	expression tag
Ala 597	-	expression tag
Ala 598	-	expression tag
Ala 599	-	expression tag
Ala 600	-	expression tag
Ile 601	-	expression tag
Asp 602	-	expression tag
Ala 603	-	expression tag
Lys 604	-	expression tag
Tyr 605	-	expression tag
Tyr 606	-	expression tag
Asn 607	-	expression tag
Ser 608	-	expression tag
Glu 609	-	expression tag
Leu 610	-	expression tag
Ala 611	-	expression tag
Ala 612	-	expression tag
Ala 613	-	expression tag
Ala 614	-	expression tag
Ala 615	-	expression tag
Ala 616	-	expression tag
Ala 617	-	expression tag
Ala 618	-	expression tag
Ala 619	-	expression tag
Ala 620	-	expression tag
Ala 621	-	expression tag
Ala 622	-	expression tag
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag
Ala 626	-	expression tag
Ala 627	-	expression tag
Ala 628	-	expression tag
Ala 629	-	expression tag

B: 2 - 342 (UniProt: Q8U338)

PDB	External Database	Details
Val 24	Glu 25	conflict
Ser 64	Glu 65	conflict
Leu 110	Val 111	conflict
-	Ser 235	deletion
-	Leu 236	deletion
-	Gly 237	deletion

O: 1 - 256 (UniProt: A0A5C0XNV9)

PDB	External Database	Details
-	Thr 235	deletion

Q: 2 - 216 (UniProt: Q8U336)

PDB	External Database	Details
-	Leu 211	deletion
Ala 217	-	expression tag
Ala 218	-	expression tag
Ala 219	-	expression tag
Ala 220	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	509555.6
Non-Polymers*	Number of molecules	2
	Total formula weight	117.4
All*	Total formula weight	509673.0

*Water molecules are not included.