7TRA

Cascade complex from type I-A CRISPR-Cas system

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS TALOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Phase Plate	VOLTA PHASE PLATE
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.3
Software	cryoSPARC,RELION,EMAN,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION