7TD3

Sphingosine-1-phosphate receptor 1-Gi complex bound to S1P

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400) In PDB	Bos taurus (cattle)	Transducin beta chain 1
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	379	43163.1	1	UniProt (B2RSH2) Pfam (PF00503) UniProt (by SIFTS) (P10824) In PDB	Rattus norvegicus (Norway rat)	Adenylate cyclase-inhibiting G alpha protein
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	R	Sphingosine 1-phosphate receptor 1	polymer	392	43938.7	1	UniProt (P21453) Pfam (PF00001) In PDB	Homo sapiens (human)	S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1
5	R	(2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate	non-polymer		379.5	1	Chemie (S1P)
6	R	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 1 - 354 (UniProt: B2RSH2)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 203	Gly 203	engineered mutation

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: 2 - 382 (UniProt: P21453)

PDB	External Database	Details
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132363.8
Non-Polymers*	Number of molecules	2
	Total formula weight	600.7
All*	Total formula weight	132964.5

*Water molecules are not included.