7T6B

Structure of S1PR2-heterotrimeric G13 signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein subunit alpha-13	polymer	362	42255.3	1	UniProt (Q14344) Pfam (PF00503) UniProt (by SIFTS) (P63096) In PDB	Homo sapiens (human)	G alpha-13,G-protein subunit alpha-13
2	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	362	39970.7	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	E	scFv16	polymer	259	27784.9	1		Mus musculus
5	R	Sphingosine 1-phosphate receptor 2	polymer	361	39902.8	1	UniProt (O95136) Pfam (PF00001) In PDB	Homo sapiens (human)	S1P receptor 2,S1P2,Endothelial differentiation G-protein coupled receptor 5,Sphingosine 1-phosphate receptor Edg-5,S1P receptor Edg-5
6	R	(2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate	non-polymer		379.5	1	Chemie (S1P)

Sequence modifications

A: 2 - 377 (UniProt: Q14344)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 4	Leu 19	conflict
Ser 6	Thr 21	conflict
Ala 7	Ser 22	conflict
Glu 8	Gly 23	conflict
Asp 9	Glu 24	conflict
Lys 10	Ala 25	conflict
Ala 11	Glu 26	conflict
Ala 12	Gln 27	conflict
Val 13	Gln 28	conflict
Glu 14	Arg 29	conflict
Arg 15	Lys 30	conflict
Met 18	Glu 33	conflict
Arg 21	Lys 36	conflict
Asn 22	Cys 37	conflict
Arg 24	Ser 39	conflict
Glu 25	Arg 40	conflict
Asp 26	Glu 41	conflict
Gly 27	Lys 42	conflict
Glu 28	Thr 43	conflict
Lys 29	Tyr 44	conflict
Ala 30	Val 45	conflict
Ala 31	Lys 46	conflict
Val 302	Ile 302	conflict
Gly 332	Asn 332	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 353 (UniProt: O95136)

PDB	External Database	Details
Asp 354	-	expression tag
Tyr 355	-	expression tag
Lys 356	-	expression tag
Asp 357	-	expression tag
Asp 358	-	expression tag
Asp 359	-	expression tag
Asp 360	-	expression tag
Lys 361	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	157774.8
Non-Polymers*	Number of molecules	1
	Total formula weight	379.5
All*	Total formula weight	158154.3

*Water molecules are not included.