7T10

CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Somatostatin receptor type 2,Kappa-type opioid receptor	polymer	408	45784.2	1	UniProt (P30874) UniProt (P41145) Pfam (PF00001)	Homo sapiens (human)
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-3	polymer	354	40584.2	1	UniProt (P08754) Pfam (PF00503)	Homo sapiens (human)	G(i) alpha-3
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37671.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (P)	Somatostatin-14	polymer	14	1641.9	1	UniProt (P61278) Pfam (PF03002)	Homo sapiens	SST-14
6	F (S)	scFv16	polymer	259	27784.9	1		Mus musculus
7	G, H, I (R, A, B)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

R: 1 - 235 (UniProt: P30874)

PDB	External Database	Details
Asp -38	-	expression tag
Tyr -37	-	expression tag
Lys -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Met -30	-	expression tag
Gly -29	-	expression tag
Gln -28	-	expression tag
Pro -27	-	expression tag
Gly -26	-	expression tag
Asn -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Ala -22	-	expression tag
Phe -21	-	expression tag
Leu -20	-	expression tag
Leu -19	-	expression tag
Ala -18	-	expression tag
Pro -17	-	expression tag
Asn -16	-	expression tag
Arg -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Ala -12	-	expression tag
Pro -11	-	expression tag
Asp -10	-	expression tag
His -9	-	expression tag
Asp -8	-	expression tag
Val -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

R: 236 - 252 (UniProt: P41145)
R: 253 - 369 (UniProt: P30874)
A: 1 - 354 (UniProt: P08754)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	161327.4
All*	Total formula weight	161327.4

*Water molecules are not included.