7T10

CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000166	molecular_function	nucleotide binding
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005886	cellular_component	plasma membrane
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0007194	biological_process	negative regulation of adenylate cyclase activity
A	0016020	cellular_component	membrane
A	0016239	biological_process	positive regulation of macroautophagy
A	0019001	molecular_function	guanyl nucleotide binding
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
A	0036064	cellular_component	ciliary basal body
A	0046039	biological_process	GTP metabolic process
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0070062	cellular_component	extracellular exosome
B	0001750	cellular_component	photoreceptor outer segment
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
B	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
B	0007265	biological_process	Ras protein signal transduction
B	0016020	cellular_component	membrane
B	0030159	molecular_function	signaling receptor complex adaptor activity
B	0044877	molecular_function	protein-containing complex binding
B	0045202	cellular_component	synapse
B	0051020	molecular_function	GTPase binding
B	0070062	cellular_component	extracellular exosome
B	0071380	biological_process	cellular response to prostaglandin E stimulus
B	0071870	biological_process	cellular response to catecholamine stimulus
B	0097381	cellular_component	photoreceptor disc membrane
B	1903561	cellular_component	extracellular vesicle
C	0005515	molecular_function	protein binding
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0016020	cellular_component	membrane
C	0031681	molecular_function	G-protein beta-subunit binding
C	0045202	cellular_component	synapse
C	0070062	cellular_component	extracellular exosome
C	0071380	biological_process	cellular response to prostaglandin E stimulus
C	0071870	biological_process	cellular response to catecholamine stimulus
P	0005179	molecular_function	hormone activity
P	0005576	cellular_component	extracellular region
R	0004930	molecular_function	G protein-coupled receptor activity
R	0004994	molecular_function	somatostatin receptor activity
R	0007186	biological_process	G protein-coupled receptor signaling pathway
R	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS00237
Number of Residues	17
Details	G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. TSIfCLTVMSIDRYLaV

Chain	Residue	Details
R	THR128-VAL144

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
B	LEU70-SER84
B	ILE157-ILE171
B	LEU285-ALA299

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	55
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	23
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	19
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	19
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	14
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Site: {"description":"Important for ligand binding"}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	27
Details	Transmembrane: {"description":"Helical; Name=1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	53
Details	Topological domain: {"description":"Cytoplasmic"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	26
Details	Topological domain: {"description":"Extracellular"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	13
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	9
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	7
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	5
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"37137991","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7X6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GVX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19478087","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2IHB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2ODE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V4Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"37137991","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2V4Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	Modified residue: {"description":"Deamidated glutamine; by Photorhabdus PAU_02230","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	1
Details	Modified residue: {"description":"ADP-ribosylcysteine; by pertussis toxin","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	1
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	48
Details	Repeat: {"description":"WD 1","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	45
Details	Repeat: {"description":"WD 2","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI31
Number of Residues	40
Details	Repeat: {"description":"WD 3","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI32
Number of Residues	41
Details	Repeat: {"description":"WD 4","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI33
Number of Residues	43
Details	Repeat: {"description":"WD 5","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI34
Number of Residues	41
Details	Repeat: {"description":"WD 6","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI35
Number of Residues	30
Details	Repeat: {"description":"WD 7","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI36
Number of Residues	1
Details	Modified residue: {"description":"Phosphohistidine","evidences":[{"source":"UniProtKB","id":"P62871","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI37
Number of Residues	13
Details	Peptide: {"description":"Somatostatin-14","featureId":"PRO_0000033088"}

Chain

Residue

Details

---