7STG

Consequences of HLA single chain trimer mutations on peptide presentation and binding affinity

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	RPA-related protein RADX peptide,Beta-2-microglobulin,MHC class I antigen chimera	polymer	423	47523.2	16	UniProt (Q6NSI4) UniProt (P16213) UniProt (A0A678ZGP6) Pfam (PF07654) Pfam (PF00129) In PDB	Homo sapiens (Human)	RPA-related and RAD51-antagonist,X-chromosome
2	B, D, F, H, J...	VHH	polymer	116	12536.8	16		Lama glama
3		water	water		18.0	104	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O, Q, S, U, W, Y, a, c, e: 2 - 9 (UniProt: Q6NSI4)

PDB	External Database	Details
Gly 10	-	linker
Gly 11	-	linker
Gly 12	-	linker
Gly 13	-	linker
Ser 14	-	linker
Gly 15	-	linker
Gly 16	-	linker
Gly 17	-	linker
Gly 18	-	linker
Ser 19	-	linker
Gly 20	-	linker
Gly 21	-	linker
Gly 22	-	linker
Gly 23	-	linker
Ser 24	-	linker

A, C, E, G, I, K, M, O, Q, S, U, W, Y, a, c, e: 25 - 123 (UniProt: P16213)

PDB	External Database	Details
Gly 124	-	linker
Gly 125	-	linker
Gly 126	-	linker
Gly 127	-	linker
Ser 128	-	linker
Gly 129	-	linker
Gly 130	-	linker
Gly 131	-	linker
Gly 132	-	linker
Ser 133	-	linker
Gly 134	-	linker
Gly 135	-	linker
Gly 136	-	linker
Gly 137	-	linker
Ser 138	-	linker
Gly 139	-	linker
Gly 140	-	linker
Gly 141	-	linker
Gly 142	-	linker
Ser 143	-	linker

A, C, E, G, I, K, M, O, Q, S, U, W, Y, a, c, e: 144 - 418 (UniProt: A0A678ZGP6)

PDB	External Database	Details
Cys 227	Tyr 108	engineered mutation
Cys 282	Ala 163	engineered mutation
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	32
	Total formula weight	960960.7
All*	Total formula weight	960960.7

*Water molecules are not included.